Location: Sunflower and Plant Biology Research
Project Number: 3060-21000-043-020-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Oct 1, 2018
End Date: Sep 30, 2020
Objective:
Objective 1: analyze allelic relationship of the DM R gene Pl17 with a new gene Pl19 using a large F2 segregation population (1,000-2,000 F2 individuals) derived from the cross between Pl17 and Pl19.
Objective 2: conduct high-resolution genetic and physical maps of the three DM R-genes, Pl17, Pl18, and Pl19 using a fine mapping strategy.
Objective 3: identify candidate genes for DM resistance.
Objective 4: develop user-friendly ‘perfect’ markers for DM R genes and release these markers to the sunflower breeding industry for MAS to rapidly incorporate the DM R-genes into finished commercial hybrids.
Approach:
We will conduct a highly integrated project that involves the following components: 1) Allelism test for DM resistance in HA 458 (Pl17) and a line carrying Pl19. Cross will be made and a large F2 population (1,000-2,000 F2 individuals) will be phenotyped for DM responses in the greenhouses, 2) Fine mapping of DM R genes, Pl17, Pl18, and Pl19, and placement of these genes to specific sequence scaffold(s), 3) Identification of candidate genes underlying each DM R genes. Functional information inferred from other species will be used to identify candidate genes. The genetic position of the DM R genes relative to candidate genes will be refined by selective genotyping and phenotyping of recombinants in the DM R gene regions, 4) Sequence analysis of DM R gene regions from the R gene donor lines. This sequence information will be used to design additional assays for the segregation of SNPs in candidate genes, 5) Development and application of SNP markers for marker-assisted selection, and 6) Interactions between DM pathogen and sunflower host.
