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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Animal Metabolism-Agricultural Chemicals Research » Research » Research Project #431175

Research Project: Airborne Transmission of Antimicrobial Resistant Bacteria and Genes, and Antibiotic Residues from Concentrated Beef Operations

Location: Animal Metabolism-Agricultural Chemicals Research

Project Number: 3060-32420-002-03-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 1, 2016
End Date: Sep 30, 2019

Objective:
1) Determine the occurrence and levels of anitbiotics in pen flooring from concentrated beef operations, 2) Determine the distance and environmental effects from the offsite transmission of airborne particulate matter from concentrated beef operations that contain antimicrobial resistant bacteria, antimicrobial resistant genes, and antibiotic residues.

Approach:
Using SASS 2300 air samplers to sample upwind and downwind air around cattle feed lots. Starting with the feedlot at the USMARC (Clay Center, NE), source material will be analyzed for antibiotic residues, antimicrobial resistant bacteria (ARB), and antimicrobial resistant genes (ARG). Air samplers will then be placed upwind of the feedlot to determine background for antibiotic residues, ARB, and ARG. Samples will attempted to be collected at different weather conditions (wind speed, rain, dry, sunny, cloudy). After upwind collections, the samplers will be placed a specific distance from the feedlot and downwind samples at similar weather conditions as upwind will be collected. After a specific number of collections, the air samplers will be moved to collect additional samples. Samples will be collected at 3 different distances downwind of the feedlot.