|GUO, WANGZHEN - NANJING AGRI UNIVERSITY
|ZHANG, TIANZHEN - NANJING AGRIC UNIVERSITY
|PAN, JIAJU - NANJING AGRIC UNIVERSITY
Submitted to: Chinese Science Bulletin
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/1/1998
Publication Date: N/A
Interpretive Summary: Cotton lines that are male sterile because of a unique cytoplasm are used to produce hybrid seed. In order for the resulting hybrids to be fertile, plants with a fertility restoring gene must be used as the pollen parent when making the hybrids. The fertility restoring gene can not be identified except when crossed with a cytoplasmic male sterile line. The identification of a molecular marker for the restorer gene allows the screening of cotton lines for the presence or absence of the restorer gene. The marker will allow the identification of lines with the restorer gene, and it will allow the manipulation of the restorer gene in the creation of new lines that contain the restorer gene.
Technical Abstract: Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertility-restoring gene (Rf1) of upland cotton. A total of 425 arbitrary 10-mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC3 segregating population of (0-613-2R X Simian No. 3). Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of (Zhong 12A-1 X 0-613-2R)F2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV-15-300 was linked with the fertility restoring gene (Rf1) with a recombination value of 13.0 +- 2.57%.