Submitted to: Hybridoma
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/30/1997
Publication Date: N/A
Citation: Interpretive Summary: Dihydrozeatin riboside is a member of the group of plant hormones called cytokinins that regulate cell division during cereal grain development. This hormone accumulates early during grain formation when the cells which eventually determine the size of the grain are being formed. This study prepared and characterized a selection of antibodies which react with this hormone and can be used to detect less than one part per billion of the hormone in plant extracts. These antibodies, and the assays developed during the course of this study, will be useful to scientists who are trying to determine the specific role of this hormone in grain development and to develop methods to regulate hormone production to stabilize grain yield when plants are subjected to environmental stresses. One of these antibodies was used during this study to measure minute quantities of this dihydrozeatin riboside in developing wheat grains.
Technical Abstract: Three monoclonal antibodies (all IgG**1) were prepared against the plant cytokinin dihydrozeatin riboside (diH[9R]Z) and characterized for suitability in the isolation and quantification of dihydrozeatin-type phytohormones. These antibodies detected as little as 5-10 pg of the homologous cytokinin when used in both competitive and noncompetitive immunoassays and each had characteristic crossreactivity with structurally-related cytokinins. One antibody was used to quantify HPLC-purified diH[9R]Z recovered from wheat tissue. The same antibody also was linked to an insoluble support for use in affinity purification of a mixture of cytokinins. These antibodies will be useful for purification and quantification of dihydrozeatin and its 9-riboside and 9-glucoside in plant tissue.