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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #403990

Research Project: Environmental and Management Influences on Animal Productivity and Well-Being Phenotypes

Location: Livestock Issues Research

Title: Metabolomic profiling of the longissimus dorsi of weaned piglets following pre- and postnatal lipopolysaccharide challenges

item DOBBINS, THOMAS - Texas Tech University
item HERNANDEZ, SEBASTIAN - Texas Tech University
item Sanchez, Nicole
item Broadway, Paul
item Carroll, Jeffery - Jeff Carroll
item MITCHELL, TY - Texas Tech University
item PETRY, AMY - Texas Tech University
item LEGAKO, JERRAD - Texas Tech University

Submitted to: Meat and Muscle Biology
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2023
Publication Date: 6/25/2023
Citation: Dobbins, T.W., Hernandez, S.M., Sanchez, N.C., Broadway, P.R., Carroll, J.A., Mitchell, T.M., Petry, A.L., Legako, J.F. 2023. Metabolomic profiling of the longissimus dorsi of weaned piglets following pre- and postnatal lipopolysaccharide challenges. Meat and Muscle Biology.

Interpretive Summary:

Technical Abstract: The objective of this study was to evaluate the metabolomic profile of the longissimus dorsi (LD) of weaned pigs following a low-dose lipopolysaccharide (LPS) challenge to gestating sows. Pregnant Camborough sows were randomly assigned to receive LPS (LPS; n= 7) at a dose of 2.5 µg/kg or saline (CON; n = 7) on 78 ± 1.8 d of gestation. At weaning (21 ± 1.3 d of age), barrows (CON: n = 17; LPS: n = 16) from each sow treatment group received a secondary LPS challenge. After the postnatal challenge, barrows (31 ± 1.3 d of age) were euthanized and tissue from the right LD was collected and snap-frozen in liquid nitrogen. Metabolites were extracted by weighing approximately 75 mg of sample and placed into a glass vial with 50 µL of ribitol and 1 mL of 80% methanol. The sample extract was dried with nitrogen gas and then reconstituted with 50 µL of a methoxyamine hydrochloride and 50 µL of N-methyl-N-trimethylsilyltrifluoroacetamide with 1% trimethylchlorosilane. Metabolites were injected into a gas chromatograph coupled with a mass spectrometer with a mass range of 50 to 650 m/z. Mass spectral data were deconvoluted, aligned, and annotated using MS-DIAL. Prior to analyses, data were median normalized, log10 transformed, and auto-scaled. Data were analyzed using MetaboAnalyst by a paired t-test. Pathway analysis was conducted and compared to the Homo sapienc pathway library. Significance for all analyses was declared at P = 0.05 and tendencies were considered at P = 0.10. Malic acid, oxalic acid, serine, and aspartic acid were increased (P = 0.039) in LPS offspring compared with CON offspring. D-panose, methyl-beta-D-galactopyranoside, inulotriose, a trisaccharide, and erythrose were increased (P = 0.044) in CON offspring compared with LPS offspring. There was a tendency for alanine, glutamic acid, inosine, dihydroxyacetone, and fumaric acid to be increased (P = 0.096) in LPS offspring compared to CON offspring. There was a tendency for 3-deoxyhexitol, cholesterol, and phosphoethanolamine to be increased (P = 0.092) in CON offspring compared with LPS offspring. Differential metabolites were used to conduct a pathway analysis, resulting in 10 pathways associated within the skeletal muscle of weaned pigs (P = 0.039) including the citric acid cycle. This study showed that an in-utero immune stimulation using LPS in gestating sows alters the metabolomic profile of weaned pigs. These results may indicate mechanisms that can alter both pre- and postnatal skeletal muscle growth and development. Furthermore, by altering the metabolome of these offspring, there is potential to alter production efficiency of growing barrows.