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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Disease and Pest Management Research Unit » Research » Publications at this Location » Publication #399604

Research Project: Knowledge Based Tools for Exotic and Emerging Diseases of Small Fruit and Nursery Crops

Location: Horticultural Crops Disease and Pest Management Research Unit

Title: Metabarcoding for plant pathologists

item SUDERMANN, MARTHA - Oregon State University
item Foster, Zachary
item CHANG, JEFF - Oregon State University
item Grunwald, Niklaus - Nik

Submitted to: Canadian Journal of Plant Pathology
Publication Type: Review Article
Publication Acceptance Date: 11/24/2023
Publication Date: 12/15/2023
Citation: Sudermann, M., Foster, Z.S., Chang, J., Grünwald, N.J. 2023. Metabarcoding for plant pathologists. Canadian Journal of Plant Pathology. 46(2):146-160.

Interpretive Summary: .

Technical Abstract: Metabarcoding is a method of choice for community analysis using high throughput sequencing. The analysis of microbial communities remains complex and is affected by myriad factors including computational, sequencing and processing errors that all need to be addressed by proper design and analysis. This article reviews the status of the field and provides guidance on proper methods including sampling, bulking and technical errors as well as analyses of experiments.