Location: Plant Stress and Germplasm Development ResearchTitle: Sequenced Mutants as a Resource for Reverse Genetics in Sorghum
|JIAO, YINPING - Texas Tech University|
|Pugh, Nicholas - Ace|
Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/31/2022
Publication Date: 11/6/2022
Citation: Xin, Z., Jiao, Y., Burow, G.B., Hayes, C.M., Chen, J., Pugh, N.A., Ware, D. 2022. Sequenced Mutants as a Resource for Reverse Genetics in Sorghum. ASA-CSSA-SSSA Annual Meeting Abstracts.
Technical Abstract: Induced mutagenesis is a powerful approach to generate variations for elucidation of gene function and create new traits for breeding. We have developed a pedigreed mutant library through chemical mutagenesis with ethylmethane sulfonate (EMS) treated seeds in sorghum and sequenced 256 randomly selected lines. The mutant library displayed many traits of significant agronomic value. This endeavor resulted in detection and cataloguing of more than 1.8 million canonical EMS-induced mutations (or variants). The variants altered the sorghum genome uniformly and bioinformatic analyses indicated that approximately 35,000 genes were affected by large consequential mutations. Mutations in genes of interest can be searched online through SorghumBase (https://www.sorghumbase.org) or through Gramene (https://www.gramene.org/). These sequenced lines are in the process of being deposited into GRIN (https://www.ars-grin.gov/) and are freely available for research.