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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #396086

Research Project: Discovery and Improvement of Traits to Enhance Sorghum as a Multiple Purpose Crop

Location: Plant Stress and Germplasm Development Research

Title: Sequenced Mutants as a Resource for Reverse Genetics in Sorghum

item Xin, Zhanguo
item JIAO, YINPING - Texas Tech University
item Burow, Gloria
item Hayes, Chad
item Chen, Junping
item Pugh, Nicholas - Ace
item Ware, Doreen

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/31/2022
Publication Date: 11/6/2022
Citation: Xin, Z., Jiao, Y., Burow, G.B., Hayes, C.M., Chen, J., Pugh, N.A., Ware, D. 2022. Sequenced Mutants as a Resource for Reverse Genetics in Sorghum. ASA-CSSA-SSSA Annual Meeting Abstracts.

Interpretive Summary:

Technical Abstract: Induced mutagenesis is a powerful approach to generate variations for elucidation of gene function and create new traits for breeding. We have developed a pedigreed mutant library through chemical mutagenesis with ethylmethane sulfonate (EMS) treated seeds in sorghum and sequenced 256 randomly selected lines. The mutant library displayed many traits of significant agronomic value. This endeavor resulted in detection and cataloguing of more than 1.8 million canonical EMS-induced mutations (or variants). The variants altered the sorghum genome uniformly and bioinformatic analyses indicated that approximately 35,000 genes were affected by large consequential mutations. Mutations in genes of interest can be searched online through SorghumBase ( or through Gramene ( These sequenced lines are in the process of being deposited into GRIN ( and are freely available for research.