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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #392407

Research Project: Nutritional Intervention and Management Strategies to Reduce Stress and Improve Health and Well-being in Cattle and Swine

Location: Livestock Issues Research

Title: Evaluation of oxidative products in receiving beef calves challenged with lipopolysaccharide (LPS) following methionine supplementation

item BARKER, SAM - Texas Tech University
item JACKSON, TREYLR - West Texas A & M University
item RICHESON, JOHN - West Texas A & M University
item Carroll, Jeffery - Jeff Carroll
item Sanchez, Nicole
item Broadway, Paul
item LEGAKO, JERRAD - Texas Tech University

Submitted to: American Society of Animal Science Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/22/2022
Publication Date: 10/1/2022
Citation: Barker, S.N., Jackson, T., Richeson, J.T., Carroll, J.A., Sanchez, N.C., Broadway, P.R., Legako, J.F. 2022. Evaluation of oxidative products in receiving beef calves challenged with lipopolysaccharide (LPS) following methionine supplementation. American Society of Animal Science Annual Meeting.

Interpretive Summary:

Technical Abstract: This study evaluated the oxidative products of beef calves challenged with LPS following methionine supplementation (L0=Control; L1=10 g/hd-1· d -1; L2=20 g/hd-1· d -1). Following an initial 40 d feeding period, steers (n=32; 379 kg±30.7 ) were challenged i.v. with LPS (0.25 µg/kg body weight) on d41. Blood was collected at -2, 0, 2, 4, 6, 8, 10, 12, 18, 24, 36 and 48h relative to the challenge at 0h. Samples were processed to isolate plasma for indicators of oxidative stress with colorimetric assays determining malondialdehyde (MDA) and reactive oxygen species (ROS) concentrations. Data were analyzed as repeated measures using the GLIMMIX procedure of SAS. Concentrations of MDA varied by time (P < 0.001), showing a large increase (P < 0.05) 2h post-LPS, then decreasing again at 4h. MDA values decreased (P < 0.05) from 6h until 18h where values returned to initial concentrations (P < 0.05). A treatment × time interaction (P < 0.001) occurred for ROS concentrations. Concentrations for all treatments were below the detectable limit at -2, 0, 10, 12, 24, 36, and 48h (P > 0.05). At 2h post-LPS, ROS was greatest in L0 cattle, least in L2, and intermediate in L1 (P < 0.05), but fell at 4h for all treatments. Values peaked at 6 h for L1 and L2 cattle, followed by a decline at 8h (P < 0.05). Control cattle were similar from 4 to 6h (P > 0.05) but peaked again at 8h (P < 0.05). These data show that oxidative stress contributes to increased ROS and secondary lipid oxidation products but may be mitigated by the inclusion of methionine supplements. These values may also reflect other immunological reactions associated with an acute endotoxin challenge.