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ARS Home » Northeast Area » Frederick, Maryland » Foreign Disease-Weed Science Research » Research » Publications at this Location » Publication #387245

Research Project: Molecular Identification, Characterization, and Biology of Foreign and Emerging Viral and Bacterial Plant Pathogens

Location: Foreign Disease-Weed Science Research

Title: Maize dwarf mosaic virus: A new multi-use tool for functional genomics in maize

item XIE, WENSHUANG - The Ohio State University
item Marty, Deemarie - Dee
item XU, JUNHUAN - The Ohio State University
item KHATRI, NITIKA - The Ohio State University
item Willie, Kristen
item MORAES, WANDERSON BUCKE - The Ohio State University
item Stewart, Lucy

Submitted to: Mid Atlantic Plant Molecular Biology Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: 8/13/2021
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. A number of potyviruses have been used as vectors for protein expression in plants. Infectious MDMV clones were engineered to express green fluorescent protein (GFP) from two genomic locations. The GFP expression and stability of insertions were examined. Engineered viruses expressing GFP were successfully transmitted by aphids (Rhopalosiphum padi). Two triple target virus-induced gene silencing constructs (VIGS) were also created and stably induced silencing. These results indicate that MDMV based vector can be used as a tool for gene function study as well potential functional trait improvement in maize