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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #385044

Research Project: Nutritional Intervention and Management Strategies to Reduce Stress and Improve Health and Well-being in Cattle and Swine

Location: Livestock Issues Research

Title: Evaluation of antioxidant capacity in receiving beef calves challenged with lipopolysaccharide (LPS)

item BARKER, SAM - Texas Tech University
item JACKSON, TREYLR - West Texas A & M University
item RICHESON, JOHN - West Texas A & M University
item Carroll, Jeffery - Jeff Carroll
item Sanchez, Nicole
item Broadway, Paul
item LEGAKO, JERRAD - Texas Tech University

Submitted to: American Society of Animal Science Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/31/2021
Publication Date: 10/8/2021
Citation: Barker, S.N., Jackson, T., Richeson, J.T., Carroll, J.A., Sanchez, N.C., Broadway, P.R., Legako, J.F. 2021. Evaluation of antioxidant capacity in receiving beef calves challenged with lipopolysaccharide (LPS). American Society of Animal Science Annual Meeting. 99(Suppl. 3):381-382.

Interpretive Summary:

Technical Abstract: The objective of this study was to evaluate antioxidant capacity in plasma of beef calves challenged with LPS. Following an initial feeding period of 40 d, steers (n = 32; 379 kg ± 30.7) were transported to the Livestock Issues Research Unit’s Bovine Immunology Research and Development Complex and challenged intravenously with LPS (0.25 µg/kg BW) on d 41. Blood samples were collected via jugular catheter at -2, 0, 2, 4, 6, 8, 10, 12, 18, 24, 36 and 48 h relative to the LPS challenge at 0 h. Blood samples were processed to isolate plasma for indicators of oxidative stress with a colorimetric assay to determine ferric reducing antioxidant power (FRAP) values via concentration of ferrous iron (µM). Data were analyzed as repeated measures using the GLIMMIX procedure of SAS. Antioxidant values did vary with time (P < 0.001) being greater (P < 0.05) at -2, 0, 2, 36, and 48 h. Antioxidant capacity was reduced at 6 h and 8 h (P < 0.05), with the least FRAP value at 8 h post-challenge. Antioxidant capacity increased (P < 0.05) again at 10 h, showing similar (P > 0.05) concentrations to those observed at 4 h. By 24 h post-challenge, plasma FRAP values increased (P < 0.05) similar to initial values at -2, 0, and 2 h. It can be inferred that oxidative stress contributes to reduced antioxidant capacity, ultimately interfering with animal growth and productivity. While these values reflect the oxidative stress response to an acute endotoxin challenge, and a subsequent recovery returning to homeostasis within 24 to 48 h, they may also correlate with other physiological and immunological indicators associated with an acute endotoxin challenge.