Submitted to: PhytoFrontiers
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/17/2021
Publication Date: 2/15/2022
Citation: Wallis, C.M., Chen, J., Perez de Leon, A.A. 2022. Mitochondrial genome resource of a grapevine strain of Trichoderma harzianum, a potential biological control agent for fungal canker diseases. Phytofrontiers. 2(2):143-146. https://doi.org/10.1094/PHYTOFR-08-21-0052-A.
Interpretive Summary: Some fungi species like Trichoderma harzianum can be used for biocontrol purposes. The strain PAR3 of T. harzianum was isolated from grapevine in central California and selected for molecular studies as part of efforts to characterize its effects against fungi that cause canker diseases in grapevines and fruit/nut trees. This research describes the mitogenome of T. harzianum PAR3. The mitogenome is the suit of genes contained in the mitochondrion, which is the part of T. harzianum PAR3 cells where most of the chemical energy powering biochemical reactions is generated. A comparative analysis of the T. harzianum PAR3 mitogenome was done through bioinformatics. Knowledge of the mitochondrial genome of T. harzianum PAR3 facilitates research to enhance our understanding of the function and evolution of fungi.
Technical Abstract: Trichoderma spp. are commonly used as bioremediation agents, biological controls, and for making biofuels. Herein, a Trichoderma harzianum strain PAR3 was isolated from grapevine roots in central California, USA. As part of a larger whole genome sequencing effort, the mitochondrial genome (mitogenome) sequence was obtained for the PAR3 strain. The mitogenome is 27,632 bp containing 14 common mitochondrial protein genes, 24 tRNA genes, two rRNA genes, and a GC% content of 27.6%. BLAST search using the PAR3 mitogenome as query against GenBank sequence database showed mitogenome MUT3171 (29,791 bp) of Trichoderma lixii was the most similar (Query Coverage = 99%; Percentage Identity=100.00%) with two major deletions, 1,274 bp and 827 bp. The PAR3 mitogenome sequence will provide a useful reference for comparing different Trichoderma isolates.