Location: Animal Parasitic Diseases LaboratoryTitle: Blue mussel (Mytilus edulis) as bioindicator of protozoan water contamination: laboratory and in situ approaches
|BIGOT-CLIVOT, AURÉLIE - Universite De Reims Champagne-Ardenne|
|LA CARBONA, STEPHANIE - Actalia Securite Des Aliment|
|CAZEAUX, CATHERINE - Actalia Securite Des Aliment|
|DURAND, LOIC - Universite De Reims Champagne-Ardenne|
|GEBA, ELODIE - Universite De Reims Champagne-Ardenne|
|LE FOLL, FRANK - Le Havre Normandy University|
|XUEREB, BENOIT - Le Havre Normandy University|
|CHALGHMI, HOUSSEM - Le Havre Normandy University|
|BASTIEN, FANNY - Universite De Reims Champagne-Ardenne|
|BONNARD, ISABELLE - Universite De Reims Champagne-Ardenne|
|PALOS LADEIRO, MELISSA - Universite De Reims Champagne-Ardenne|
|ESCOTTE-BINET, SANDY - Universite De Reims Champagne-Ardenne|
|AUBERT, DOMINIQUE - Universite De Reims Champagne-Ardenne|
|VILLENA, ISABELLE - Universite De Reims Champagne-Ardenne|
|GEFFARD, ALAIN - Universite De Reims Champagne-Ardenne|
Submitted to: Journal of Applied Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/15/2021
Publication Date: 6/21/2021
Citation: Bigot-Clivot, A., La Carbona, S., Cazeaux, C., Durand, L., Geba, E., Le Foll, F., Xuereb, B., Chalghmi, H., Dubey, J.P., Bastien, F., Bonnard, I., Palos Ladeiro, M., Escotte-Binet, S., Aubert, D., Villena, I., Geffard, A. 2021. Blue mussel (Mytilus edulis) as bioindicator of protozoan water contamination: laboratory and in situ approaches. Water Research. 1-11. https://doi.org/10.1111/jam.15185.
Interpretive Summary: Ingestion of food and water fecally-contaminated with pathogenic protozoa (Cyclospora, Cystoisospora, Giardia, Cryptosporidium, Toxoplasma) can cause severe gastrointestinal disorders in humans and animals. Detection of these protozoans in water or fruits and vegetables is difficult. To overcome this, scientists used a marine bivalve Mytilus edulis (blue mussel), as a means to monitor water contamination. This approach was chosen because the muscle can concentrate and retain, for weeks, such protozoa, allowing them to provide information about contamination during a prolonged interval. The method was determined to detect even very low levels of contamination. This research was completed in 2019, before closure of Toxoplasma research at USDA. These findings will be of interests to public health workers, water quality officials, biologists, epidemiologists, and parasitologists.
Technical Abstract: The protozoan parasites Cryptosporidium spp., Giardia duodenalis and Toxoplasma gondii are identified as public health priorities since they are recognized as major parasites of waterborne outbreaks. It is therefore urgent to improve analytical tools for the detection of these biological contaminants for monitoring of water bodies. Our researches are focused on the marine bivalve Mytilus edulis (blue mussel). Firstly, mussels were exposed to three concentrations of G. duodenalis cysts and C. parvum or T. gondii oocysts for 21 days followed by 21 days of depuration in clear water. Secondly, wild marine blue mussels were collected along the Northwest coast of France. Our results highlighted that the blue mussels were not only able to represent the water contamination level in laboratory conditions but also in field since 8 of 9 sites were found to be positive to at least one protozoan species. Thus, M. edulis seems to be a relevant tool to bioaccumulate and retain protozoan (oo)cysts in laboratory and in field conditions indicates the potential applicability of this species for biological monitoring of seawater contamination with these pathogens.