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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Parasitic Diseases Laboratory » Research » Publications at this Location » Publication #375783

Research Project: Molecular Approaches to Control Intestinal Parasites that Affect the Microbiome in Swine and Small Ruminants

Location: Animal Parasitic Diseases Laboratory

Title: Transcriptome analysis reveals the protective role of fructo-oligosaccharide in colonic mucosal barriers in exercise-induced stressed mice

item CAO, WANXIU - Ocean University Of China
item CHIN, YAOXIAN - Hainan University
item Li, Robert
item XUE, CHANGHU - Ocean University Of China
item WANG, YUMING - Ocean University Of China
item TANG, QINGJUAN - Ocean University Of China

Submitted to: Food and Function
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/31/2021
Publication Date: 4/22/2021
Citation: Cao, W., Chin, Y., Li, R.W., Xue, C., Wang, Y., Tang, Q. 2021. Transcriptome analysis reveals the protective role of fructo-oligosaccharide in colonic mucosal barriers in exercise-induced stressed mice. Food and Function.

Interpretive Summary: Accumulating evidence has demonstrated numerous health benefits of physical exercise. However, intense exercise often causes fatigue, which is characterized by abnormal energy metabolism. Previous studies have attempted to identify prebiotics or natural products to alleviate fatigue by improving glycogen synthesis and mitochondrial function in muscles. Fructo-oligosaccharides (FOS) as a prebiotic are conducive to restore gut microbial dysbiosis induced by exercise stress and promote the growth of several beneficial microbes. In this study, we examined the mechanisms by which FOS alleviated the exercise induced fatigue using cutting-edge genomics and microbiome technologies. Our results provide evidence that FOS improve energy acquisition in animal models via the modulation of gut microbial interactions and should facilitate the design of optimal synbiotics for animal and human health.

Technical Abstract: Functional prediction improves our understanding of novel activities of pre- and pro-biotics. However, the predictions need to be validated in relevant animal models. In the present study, the phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) algorithm was used to to demonstrate that fructo-oligosaccharides (FOS) promoted the production of short chain fatty acids (SCFAs). Colonic transcriptome analysis also revealed that FOS significantly upregulated host genes related to energy synthesis. Together, our results suggest that FOS improved host energy acquisition and common fatigue related indices, such as glucogen storage in liver and muscle, through modulating the composition and function of the gut microbial community. Our findings should facilitate the design of next generation synbiotics.