Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/19/2019
Publication Date: 9/5/2019
Citation: Burbank, L.P., Wei, W. 2019. Broad host-range plasmids for constitutive and inducible gene expression in the absence of antibiotic selection. Microbiology Resource Announcements. 8(36). https://doi.org/10.1128/MRA.00769-19.
Interpretive Summary: Plasmid vectors are a valuable research tool for characterizing bacterial gene function but there is a limited range of plasmids that are functional in non-model bacterial species. This resource announcement describes a set of plasmids that were developed for microbiology research applications and can be used in a wide range of bacterial species. These plasmids have been modified for increased stability in bacterial populations under environmental conditions and for controllable gene expression.
Technical Abstract: Plasmid vectors are a valuable research tool for characterizing bacterial gene function but there is a limited range of plasmids that are functional in non-model bacterial species. Described here are a set of broad host range plasmids modified for stability in absence of antibiotic selection and for gene expression manipulation.