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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #365172
Research Project: Identification of Novel Management Strategies for Key Pests and Pathogens of Grapevine with Emphasis on the Xylella Fastidiosa Pathosystem

Location: Crop Diseases, Pests and Genetics Research

Title: Broad host-range plasmids for constitutive and inducible gene expression in the absence of antibiotic selection

Author
item Burbank, Lindsey
item Wei, Wei

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/19/2019
Publication Date: 9/5/2019
Citation: Burbank, L.P., Wei, W. 2019. Broad host-range plasmids for constitutive and inducible gene expression in the absence of antibiotic selection. Microbiology Resource Announcements. 8(36). https://doi.org/10.1128/MRA.00769-19.
DOI: https://doi.org/10.1128/MRA.00769-19

Interpretive Summary: Plasmid vectors are a valuable research tool for characterizing bacterial gene function but there is a limited range of plasmids that are functional in non-model bacterial species. This resource announcement describes a set of plasmids that were developed for microbiology research applications and can be used in a wide range of bacterial species. These plasmids have been modified for increased stability in bacterial populations under environmental conditions and for controllable gene expression.

Technical Abstract: Plasmid vectors are a valuable research tool for characterizing bacterial gene function but there is a limited range of plasmids that are functional in non-model bacterial species. Described here are a set of broad host range plasmids modified for stability in absence of antibiotic selection and for gene expression manipulation.