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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #362125

Research Project: Identification of Novel Management Strategies for Key Pests and Pathogens of Grapevine with Emphasis on the Xylella Fastidiosa Pathosystem

Location: Crop Diseases, Pests and Genetics Research

Title: Microbiomes of Xylella fastidiosa infected grapevine in California

item Van Horn, Christopher
item DAI, ZEHAN - South China Agricultural University
item Sisterson, Mark
item Chen, Jianchi

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/4/2019
Publication Date: 10/2/2019
Citation: Van Horn, C.R., Dai, Z., Sisterson, M.S., Chen, J. 2019. Microbiomes of Xylella fastidiosa infected grapevine in California. American Phytopathological Society Abstracts. 109:S2.1.

Interpretive Summary:

Technical Abstract: Xylella fastidiosa (Xf) causing Pierce’s Disease (PD) of grapevine is known to associate with endophytes (microbiome) during infection and pathogenicity development. The increase of Xf genetic resources in online databases, along with improved sequencing and genetic analysis technology, has provided a framework to study the PD microbiome. In this study, DNA was extracted from a PD symptomatic grapevine inoculated with Xf strain Stag’s Leap in greenhouse (Greenhouse Grape - GG), and a PD symptomatic grapevine from a vineyard in Bakersfield, CA (Field Grape - FG). Illumina HiSeq was used on GG and generated 190 M 100-bp short sequence reads. Illumina MiSeq was used on FG and generated 39 M 250-bp short sequence reads. Percentages of Xf reads were 1.7 for GG, and 0.5 for FG. Genomic variations of Xf under different conditions (field, greenhouse, sampling time) were studied. The microbiomes of GG and FG were analyzed to determine the major taxonomic groups of bacteria and fungi through metagenomic approach with the help of Kaiju software. The most abundant bacterial genera present in GG was Xf. The most abundant bacterial genera present in FG was Paenarthrobacter, with Xf ranking third. Many endophytic bacteria such as Enterococcus, Staphylococcus, and Paraburkholderia were observed. These results provide new information on grape microbiome associated with Xf infection under greenhouse and field environment.