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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #362120

Research Project: Breeding Prunus and Vitis Scions for Improved Fruit Quality and Durable Pest Resistance

Location: Crop Diseases, Pests and Genetics Research

Title: Genotypic diversity among Botrytis Cinerea isolates from California

item Delong, Jeffery - Jeff
item Saito, Seiya
item Xiao, Chang-Lin
item Naegele, Rachel

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 9/30/2019
Publication Date: 10/1/2019
Citation: Delong, J.A., Saito, S., Xiao, C., Naegele, R.P. 2019. Genotypic diversity among Botrytis Cinerea isolates from California. Phytopathology. 109(10):171.

Interpretive Summary:

Technical Abstract: Botrytis cinerea, the causal agent of grey mold, has high genetic diversity and a broad host range. In grape and Prunus spp., Botrytis causes a fruit rot, and fungicide sprays are routinely used to prevent loss pre- and post harvest. Five hundred thirty-five isolates of B. cinerea collected from grape and Prunus spp. in 2012, 2016, and 2017 were genotyped using 18 microsatellite markers and transposable elements (TE) Boty and Flipper. Only nine markers were considered informative and retained for the final analyses. Four hundred ninety-eight of the isolates had been tested for resistance to six or seven different fungicides, representing 5 classes. After clone correction, 323 multi-locus genotypes (MLG) groups were retained across the three years, and four genetic sub-populations were detected. High levels of clonality were observed across the dataset. Significant pairwise differentiation was detected among years, locations, TE composition, and host. However, most of the differentiation observed was within a subpopulation, and not between subpopulations. No differentiation was detected among fungicide resistance isolates when compared against individual products. When resistance to different numbers of fungicides were compared regardless of the chemistry, little differentiation was detected among resistance to two fungicides and three or four fungicide chemistries.