Location: Bioproducts ResearchTitle: Lactic acid bacteria for growth promotion and fungal disease management in crops
|NADEEM, AHMAD - US Department Of Agriculture (USDA)|
|BIBI, NOREEN - Pakistan Institute Of Engineering And Applied Sciences (PIEAS)|
|ARSHAD, RUBINA - Pakistan Institute Of Engineering And Applied Sciences (PIEAS)|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/28/2019
Publication Date: 6/23/2019
Citation: Nadeem, A., Bibi, N., Arshad, R., Lee, C.C. 2019. Lactic acid bacteria for growth promotion and fungal disease management in crops. Meeting Abstract. [abstract]. ASM Microbe 2019 conference, San Francisco, CA. June 20-24, 2019.
Technical Abstract: Lactic acid bacteria (LAB) are generally recognized as safe microorganisms. They produce many secondary metabolites including bioactive compounds and organic acids which express antagonistic activity against microbial pathogens of plants. The present study is designed to explore the potential of indigenous LAB and their metabolites for growth promotion and disease control in chickpea. For this purpose, LAB were isolated from different plant sources (cotton and rice leaves) on selective and differential media such as Lactobacillus plantarum specific medium, de Man Rogosa Sharpe (MRS) medium and LB medium. Bacterial isolates were identified on the basis of morphological and biochemical characteristics and further confirmed through 16S-rDNA sequence analysis. Physical (gamma radiation) and chemical (N-methyl-N'-nitro-N-nitrosoguanidine) mutagenesis of LAB isolates was then carried out to observe the effects on bioactive compounds production. The mutant isolates were analyzed for characterization of bioactive compounds by UV-Vis spectroscopy, TLC and HPLC techniques. The bioactivity and antagonistic potential of whole cells or cell-free culture supernatant of hyper-producing mutant isolates will be assessed in vitro by well diffusion and dual culture assay. The LAB isolates with antagonistic activity were then tested in vivo by seed treatment and under field conditions. The indigenous isolates showed signs for growth promotion and as biocontrol agent in chickpea by restricting Fusarium growth.