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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Parasitic Diseases Laboratory » Research » Publications at this Location » Publication #360574

Research Project: Molecular Approaches to Control Intestinal Parasites that Affect the Microbiome in Swine and Small Ruminants

Location: Animal Parasitic Diseases Laboratory

Title: The caprine abomasal microbiome

item Li, Robert
item Urban, Joseph

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Abstract Only
Publication Acceptance Date: 2/6/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Haemonchus contortus is one of the most injurious helminth parasite for small ruminants. In this study, we characterized the impact of H. contortus infection on the caprine abomasal microbiome. The dataset we obtained included 20 goats with a total of 4,523,575 paired-end sequences (2x300bp). Fourteen parasite naive goats were inoculated with 5,000 H. contortus infective larvae and followed for 50 days. Six age-matched naïve goats served as uninfected controls. Reduced bodyweight gain and a significant increase in the abosamal pH was observed in infected goats compared to uninfected controls. Infection also increased the bacterial load while reducing the abundance of the Archaea in the abomasum but did not appear to affect microbial diversity. Nevertheless, the infection altered the abundance of approximately 19% of the operational taxonomic units detected per sample. Up to 30 taxa displayed a significantly different abundance between control and infected goats. Furthermore, the infection resulted in a distinct difference in the microbiome structure. Eight biological pathways were significantly affected by infection. In addition, H. contortus induced changes in butyrate producing bacteria could regulate mucosal inflammation and tissue repair. Our results provided insight into physiological consequences of helminth infection in small ruminants and could facilitate the development of novel control strategies to improve animal and human health.