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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #359778

Research Project: Non-antibiotic Strategies to Control Enteric Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: Clostridium perfringens induces IL-10 secretion in chicken intestinal epithelial cells and necrotic enteritis broiler chicken

item LEE, YOUNGSUB - US Department Of Agriculture (USDA)
item KIM, WOOHYUN - US Department Of Agriculture (USDA)
item Lillehoj, Hyun

Submitted to: American Association of Avian Pathologist
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The Aim of this study was to assess the production level of interleukin (IL)-10 in Clostridium perfringens (CP)-stimulated intestinal epithelial cells (IECs) and CP-infected chickens using an antigen capture ELISA based on mouse monoclonal antibodies (mAbs) specific against chicken IL-10. Chicken IECs were stimulated with CP toxin in the absence or presence of antibodies (a-toxin or NetB) for 18'h. Expression of chicken IL-10 and IL-6 was monitored by quantitative real-time polymerase chain reaction. Serum and jejunum samples were collected from CP-infected chickens at 9 days post-infection and expression of IL-10 and IL-6 was analyzed. IL-10 production was detected by antigen capture ELISA in chicken IECs stimulated with CP and in serum samples collected from CP-infected birds. The mRNA levels were consistent with the results of antigen capture ELISA. CP induced IL-10 production in chicken IECs. Increased IL-10 production was detected in CP-stimulated chicken IECs and in serum collected from CP-infected birds, using antigen capture ELISA. Antigen capture ELISA could be a useful tool to monitor IL-10 production in chicken disease.