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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #359774

Research Project: Non-antibiotic Strategies to Control Enteric Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: Development of poultry-specific immune reagents

item Lillehoj, Hyun
item KIM, WOOHYUN - US Department Of Agriculture (USDA)
item Li, Charles
item SUN, ZHIFENG - US Department Of Agriculture (USDA)
item LEE, YOUNGSUB - US Department Of Agriculture (USDA)
item CHAUDHARI, ATUL - US Department Of Agriculture (USDA)
item GOO, DOYUN - US Department Of Agriculture (USDA)
item Panebra, Alfredo
item HONG, YOUNG - Chung-Ang University
item LABRESH, JOANNA - Kingfisher Biotech, Inc

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The goals of this NIFA grant are 1) to identify chicken immune molecules, particularly cytokines, chemokines and cell surface markers, and characterize their function, and 2) to develop monoclonal antibodies (mAbs) for assay development. Cloning of chicken genes (20 in total) were carried out using the number of sets of primers and recombinant proteins were expressed in E. coli, mammalian cells or yeast. Hybridomas which secrete mAbs against 20 target chicken proteins were developed. For functional characterization of the recombinant protein and mAbs, several assays have been conducted including ELISA, immunohistochemistry, Western blot, flow cytometry, qPCR, cell proliferation, and nitric oxide assay. All the target we selected have shown to have critical functions in host defense against pathogens and all recombinant proteins expressed have met the quality standard for immunization in mice for mAbs production. In summary, we have expressed 20 proteins (11 from yeast, 9 from E. coli) and 5 proteins expressed from mammalian system for mAb development and functional study. Twenty target proteins consist of 13 cytokines, 4 chemokines, 1 surface receptor, and others. For mAb development, the progress is at various stages with 3 finished, 9 in characterization, 4 in production, and 2 in screening. The mAbs developed in this study represent new sets of immune reagents which are species-specific for poultry.