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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #355718

Research Project: Genomics of Livestock Pests

Location: Livestock Arthropod Pests Research

Title: Assembled draft genome of the horn fly, Haematobia irritans

Author
item Guerrero, Felicito - Felix
item Bendele, Kylie
item Foil, Lane - Louisiana State University Agcenter
item Schilkey, Faye - National Center For Genome Resources
item Konganti, Kranti - Texas A&M University

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 11/7/2017
Publication Date: 5/4/2018
Citation: Guerrero, F., Bendele, K.G., Foil, L.D., Schilkey, F., Konganti, K. 2018. Assembled draft genome of the horn fly, Haematobia irritans. National Center for Biotechnology Information (NCBI). PGFW01000000.

Interpretive Summary: Next-generation DNA sequencing technology was used to sequence the genome of the horn fly, Haematobia irritans. The biological material was collected from pooled male and female unfed adult flies from the Kerrville in vitro susceptible reference colony maintained at the USDA-ARS Knipling-Bushland U. S. Livestock Insects Research Laboratory in Kerrville, TX, USA. A total of 1,143,537,531 nucleotides were assembled using SOAPdenovo Ver. 2, producing a draft quality assembly that consisted of 76,616 scaffolds with N50 = 23,099. The assembly has been deposited at NCBI under the BioProject accession number PRJNA30967 and the BioSample accession number SAMN07830356. The assembled genome of this Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession PGFW00000000. The version described here is version PGFW01000000. USDA is an equal opportunity employer.

Technical Abstract: Sanger DNA sequencing technology was used to sequence 4 clones from a BAC library synthesized from genomic DNA of the cattle tick, Rhipicephalus microplus. The DNA was derived from 14 day old eggs from the Deutsch Texas outbreak strain reared at the USDA-ARS Cattle Fever Tick Research Laboratory, Edinburg, TX. Each corresponding dataset of assembled sequence reads has been deposited at NCBI under the BioProject accession number PRJNA412317 and Nucleotide Database for Genomic DNA Accession Numbers MH158989, MH158990, MH158991, and MH158992, containing 103,993, 93,110, 104,603, and 89,876 nucleotides, and 6, 9, 12, and 4 unordered sequences, respectively.