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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #355717

Research Project: Genomics of Livestock Pests

Location: Livestock Arthropod Pests Research

Title: Sequencing of 4 BAC clones from the Deutsch strain of cattle ticks, Rhipicephalus microplus using the Sanger method

Author
item Bendele, Kylie
item Guerrero, Felicito - Felix

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 4/4/2018
Publication Date: 4/4/2018
Citation: Bendele, K.G., Guerrero, F. 2018. Sequencing of 4 BAC clones from the Deutsch strain of cattle ticks, Rhipicephalus microplus using the Sanger method. National Center for Biotechnology Information (NCBI). PRJNA412317, MH158989, MH158990, MH158991, MH158992.

Interpretive Summary: Classic Sanger DNA sequencing technology was used to sequence 4 large BAC clones containing parts of the genome of the cattle tick, Rhipicephalus microplus. The DNA was derived from 14 day old eggs from the Deutsch Texas outbreak strain reared at the USDA-ARS Cattle Fever Tick Research Laboratory, Edinburg, TX. Each corresponding dataset of assembled sequence reads has been deposited at NCBI under the BioProject accession number PRJNA412317 and Nucleotide Database for Genomic DNA Accession Numbers MH158989, MH158990, MH158991, and MH158992.

Technical Abstract: Sanger DNA sequencing technology was used to sequence 4 clones from a BAC library synthesized from genomic DNA of the cattle tick, Rhipicephalus microplus. The DNA was derived from 14 day old eggs from the Deutsch Texas outbreak strain reared at the USDA-ARS Cattle Fever Tick Research Laboratory, Edinburg, TX. Each corresponding dataset of assembled sequence reads has been deposited at NCBI under the BioProject accession number PRJNA412317 and Nucleotide Database for Genomic DNA Accession Numbers MH158989, MH158990, MH158991, and MH158992, containing 103,993, 93,110, 104,603, and 89,876 nucleotides, and 6, 9, 12, and 4 unordered sequences, respectively.