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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #355714

Research Project: Genomics of Livestock Pests

Location: Livestock Arthropod Pests Research

Title: Assembled transcriptome of the shoot of the giant reed, Arundo donax

Author
item BARRERO, ROBERTO - Murdoch University
item Guerrero, Felicito - Felix
item MOOLHUIJZEN, PAULA - Murdoch University
item Goolsby, John
item Tidwell, Jason
item BELLGARD, STANLEY - Landcare Research
item BELLGARD, MATTHEW - Murdoch University

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 9/11/2014
Publication Date: 6/28/2015
Citation: Barrero, R.A., Guerrero, F., Moolhuijzen, P., Goolsby, J., Tidwell, J.P., Bellgard, S.E., Bellgard, M.I. 2015. Assembled transcriptome of the shoot of the giant reed, Arundo donax. National Center for Biotechnology Information (NCBI). GBRH01000000.

Interpretive Summary: Next-generation DNA sequencing technology was used to sequence the transcriptome from the shoot stage of the perennial grass species, Arundo donax. The biological material was collected from an invasive genotype along the Rio Grande River bordering Texas and Mexico. A total of 181,972,782 pair-end raw reads were produced and assembled into 27,491 high confidence transcripts. The raw read sequences have been deposited at NCBI under the BioProject accession number PRJNA256910, the BioSample accession number SAMN02943944, and the Sequence Read Archive accession SRR1559299. The pooled and assembled transcriptome has been deposited at DDBJ/EMBL/GenBank to the Transcriptome Shotgun Assembly database under the accession GBRH00000000. The version described here is the first version, GBRH01000000. USDA is an equal opportunity employer.

Technical Abstract: Illumina HiSeq technology was used to sequence the transcriptome from the shoot stage of the perennial grass species, Arundo donax. The biological material was collected from an invasive genotype along the Rio Grande River bordering Texas and Mexico. A total of 181,972,782 pair-end raw reads were produced and assembled into 283,603 contigs. Ultimately, 27,491 and 75,590 were classified as high and low confidence transcripts, respectively. The raw read sequences have been deposited at NCBI under the BioProject accession number PRJNA256910, the BioSample accession number SAMN02943944, and the Sequence Read Archive accession SRR1559299. The pooled and assembled transcriptome has been deposited at DDBJ/EMBL/GenBank to the Transcriptome Shotgun Assembly database under the accession GBRH00000000. The version described here is the first version, GBRH01000000. USDA is an equal opportunity employer.