|KIM, WOOHYUN - US Department Of Agriculture (USDA)|
|LEE, YOUNGSUB - US Department Of Agriculture (USDA)|
Submitted to: Poultry Science Meeting
Publication Type: Other
Publication Acceptance Date: 3/21/2018
Publication Date: N/A
Technical Abstract: Interleukin (IL)-10 is an anti-inflammatory cytokine which regulates host innate immune response. Besides T cells which are the major source of IL-10, the intestinal epithelial cells (IECs) also have been shown to express IL-10 to maintain the epithelial integrity in mammals. In chickens, there is no reported study on the expression of IL-10 in chicken IECs. In this study, we treated chicken IECs with Clostridium perfringens (CP), a causative agent of necrotic enteritis (NE). NE is caused by virulent strains of CP which induce severe local inflammatory response in chicken intestinal mucosa. In the present study, chicken IECs were stimulated with either heat-killed CP (HK-CP) or supernatant from CP culture which were collected at different time points and dosage levels. The expressions of chicken IL-10 and chicken IL-6 (an inflammatory marker) were monitored by quantitative real-time polymerase chain reaction (qRT-PCR). In addition, we developed an antigen capture assay using the newly developed mouse monoclonal antibodies against chicken IL-10, to quantify IL-10 protein production in CP-stimulated IECs. Compared to non-stimulated control, the mRNA expressions of IL-10 were highly induced in IECs stimulated with HK-CP and the supernatant from CP culture. Interestingly, the supernatant from CP culture contained CP toxins and induced higher level of IL-10 compared to HK-CP indicating that CP toxins may play a role in IL-10 production. Additionally, the protein concentration of IL-10 measured by the antigen capture assay agrees with the mRNA expression. The results of present study provide a new insight on early host immune response to CP in NE infection.