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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #350872

Research Project: Non-antibiotic Strategies to Control Enteric Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: "Interlaeukin-34 regulates Th1 and Th17 cytokine production by activating multiple signaling pathways through CSF-1R in chicken cell lines"

item TROUNG, ANH - Chung-Ang University
item HONG, YEOJIN - Chung-Ang University
item LEE, JANGGEUN - Chung-Ang University
item LEE, KYUNGBAEK - Chung-Ang University
item KIL, DONG YONG - Chung-Ang University
item Lillehoj, Hyun
item HONG, YEONG HO - Chung-Ang University

Submitted to: Oncotarget
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/4/2018
Publication Date: 9/11/2018
Citation: Troung, A.D., Hong, Y., Lee, J., Lee, K., Kil, D., Lillehoj, H.S., Hong, Y. 2018. "Interlaeukin-34 regulates Th1 and Th17 cytokine production by activating multiple signaling pathways through CSF-1R in chicken cell lines". Oncotarget. 19(9):2710 E1665.

Interpretive Summary: Limited information on poultry immune system hinders rapid progress in developing effective disease control strategies against many poultry infections. In this paper, ARS scientists collaborated with scientists in South Korea to identify new immune molecule of poultry immune system which plays an important role in controlling many aspects of T-lymphocyte response in chickens. The newly discovered cytokine which is secreted by T-lymphocytes is called interleukin (IL)-34 and is expressed in several cell types in chickens. In mammals, IL-34 induces many cytokines which regulate inflammatory response. The results of this study established that chicken IL-34 enhance innate and adaptive immune responses through its action on intracellular signaling pathways. This information is important in the study of host-pathogen immunobiology.

Technical Abstract: Interleukin-34 (IL-34) is a newly recognized cytokine with functions similar to macrophage colony-stimulating factor 1, which is expressed in macrophages and fibroblast cells where it induces cytokine production; however, the mechanism of chicken IL-34 (chIL-34) signaling has not been identified to date. The aim of this study was to analyze the signal transduction pathways and specific biological functions associated with chIL-34 in chicken macrophage (HD11) and fibroblast (OU2) cell lines. We found that IL-34 is a functional ligand for the CSF-1 receptor (CSF-1R) in chicken cell lines. Treatment with chIL-34 increased the expression of Th1 and Th17 cytokines through phosphorylation of tyrosine and serine residues in JAK2 and TYK2, STAT1 and STAT3, and SHP-2, which also led to phosphorylation of NF-'B1, TAK1, MyD88, SOCS1, and ERK1/2. Taken together, these results suggest that chIL-34 functions by binding to CSF-1R and activating the JAK/STAT, NF-'B, and MAPK signaling pathways; these signaling events modulate cytokine expression and suggest roles for chIL-34 in innate and adaptive immunity.