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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #350242
Research Project: Non-antibiotic Strategies to Control Enteric Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: Development and characterization of chicken CD127-cpecific antibodies

Author
item ZHIFENG, SUN - US Department Of Agriculture (USDA)
item ZHAO, HONGYAN - US Department Of Agriculture (USDA)
item KIM, WOOHYUN - US Department Of Agriculture (USDA)
item Panebra, Alfredo
item Lillehoj, Hyun
item XIANYU, ZHEZI - US Department Of Agriculture (USDA)
item GU, CHANGQIN - US Department Of Agriculture (USDA)
item Yan, Xianghe
item Li, Charles

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Research in avian immunology has been significantly hampered by lack of effective immunological reagents in birds cross-reactive with mammalian orthologs and lack of sensitive assay for a long time. To better serve the avian immunology community, monoclonal and polyclonal antibodies specific for avian molecules of Clusters of Differentiation (CD) are being developed to promote thorough research on avian infection and immunity. In this study, chicken CD127 antibodies was developed and characterized. CD127, also named interleukin-7 receptor (IL-7R), is expressed on various cell types, including native and memory T-cells and many other, and plays critical roles in differentiation and activation of T lymphocytes. A 678 bp extracellular region of chicken CD127 gene was cloned in pET8a(+) vector and expressed in BL21-AI™ E. coli. The expressed CD127 protein was around 30 kDa and crossed-reacted with monoclonal antibody specific for human CD127 (clone G-11). The protein was then immunized in BALB/c mice. After 3 immunizations, murine sera could detect a band of 30 KDa from a protein expressed in E. coli, and detect the protein expressed on chicken T cell line (CU205) by flow cytometry as well. Flow cytometric analysis of cells from two-week-old chickens showed that CD127-positive populations were detected in spleen and thymus with such murine sera as polyclonal antibodies, and in thymus detected when using monoclonal antibody clone G-1. Availability of these antibodies for CD127 and other CD molecules will dramatically facilitate the commercialization of poultry immune reagents, greatly benefit the avian immunology community and promote the poultry health and production, where it will have a significant global impact in multiple sectors (i.e. agriculture and human healthcare).