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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #348679

Research Project: Genomics of Livestock Pests

Location: Livestock Arthropod Pests Research

Title: Rhipicephalus microplus dataset of nonredundant raw sequence reads from 454 GS FLX sequencing of Cot-selected (Cot = 660) genomic DNA

Author
item Bendele, Kylie
item Guerrero, Felicito - Felix

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 11/7/2017
Publication Date: 11/7/2017
Citation: Bendele, K.G., Guerrero, F. 2017. Rhipicephalus microplus dataset of nonredundant raw sequence reads from 454 GS FLX sequencing of Cot-selected (Cot = 660) genomic DNA. National Center for Biotechnology Information (NCBI). GenBank Accession Sequence Submissions PRJNA412317, SAMN07988077, SRS2669643.

Interpretive Summary: The genome of the cattle tick, Rhipicephalus microplus, is very complex and large. Thus, to facilitate genome sequencing, a selection technique known as Cot selection was used to isolate a less complex fraction of the genome for sequencing using 454 GS FLX technology. These sequences have been deposited at NCBI under the BioProject, BioSample, and Short Read Archive (SRA) accession numbers PRJNA412317, SAMN07988077, and SRS2669643, respectively. USDA is an equal opportunity employer.

Technical Abstract: A reassociation kinetics-based approach was used to reduce the complexity of genomic DNA from the Deutsch laboratory strain of the cattle tick, Rhipicephalus microplus, to facilitate genome sequencing. Selected genomic DNA (Cot value = 660) was sequenced using 454 GS FLX technology, resulting in 356.1 Mbp of sequence data after quality filtering. These sequences have been deposited at NCBI under the BioProject, BioSample, and Short Read Archive (SRA) accession numbers PRJNA412317, SAMN07988077, and SRS2669643, respectively. USDA is an equal opportunity employer.