Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2017
Publication Date: 12/15/2017
Citation: Chen, J., Van Horn, C.R. 2017. Identification of a low copy number plasmid in Xylella fastidiosa Strain Stag’s Leap. CDFA Pierce's Disease Control Program Research Symposium. p. 119-125.
Technical Abstract: Xylella fastidiosa (Xf) causes Pierce’s Disease (PD) in grapevine. The Stag’s Leap strain is known for its high virulence level and is a model for PD research. Research on Xf has been difficult due to its nutritional fastidiousness. One difficult research issue is the low copy number plasmid. Plasmids are circular extrachromosomal genetic elements associated with bacterial environmental adaptation, including virulence. In this study, a low copy number plasmid, pXFSL21, was identified and characterized using a next-generation sequencing (NGS) approach. Plasmid pXFSL21 (21,665 bp) contains 28 annotated genes including two predicted antibiotic resistance genes, acrA/B, encoding a multidrug efflux pump system. Under the experimental conditions in this study, pXFSL21 likely existed as a single copy per genome and had a potential of integration into the host chromosome. These results set the base for further studies on Xf-host interactions.