|TRUONG, ANH - Chung-Ang University|
|HOANG, CONG THANH - Chung-Ang University|
|HONG, YEOJIN - Chung-Ang University|
|LEE, JANGGEUN - Chung-Ang University|
|LEE, KYUNGBAEK - Chung-Ang University|
|HONG, YEONG - Chung-Ang University|
Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2017
Publication Date: 12/20/2017
Citation: Truong, A.D., Hoang, C., Hong, Y., Lee, J., Lee, K., Lillehoj, H.S., Hong, Y.H. 2017. Dataset on characterization of recombinant interleukin-23alpha, IL-12p40 and IL-23 complex protein, which activates JAK-STAT signaling pathway in chicken cell line using immunocytochemical. Data in Brief. 16:799-805. https://doi.org/10.1016/j.dib.2017.12.008.
Interpretive Summary: This is a data submission for research article entitled “Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex” where we demonstrated that the chicken interleukin (IL)-23a, IL-12p40, and IL-23 complex regulates Th1, Th17, and Treg cytokine production through various cell surface receptors. Using various chicken cell lines, underlying signaling pathways are determined. This summary will enhance our understanding of complex immune regulation.
Technical Abstract: The data herein is related to the research article entitled “Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex”  where we demonstrated that the chicken interleukin (IL)-23a, IL-12p40, and IL-23 complex regulates Th1, Th17, and Treg cytokine production through heterodimer receptors as well as a homo-dimer receptor consisting of IL-12Rß1 and IL-23R, and activates the JAK/STAT signaling pathways. Here, we evaluated the effects of the recombinant chicken IL-23a, IL-12p40, and IL-23 complex protein on cell proliferation and nitric oxide (NO) production in chicken macrophage (HD11) and CU91 T cell lines. In addition, the expression of IL-6, IL-17A, and interferon-' mRNA were up-regulated in vivo and in vitro. Moreover, treatment with the chicken IL-23a, IL-12p40, and IL-23 complex activated phosphorylation of tyrosine and serine residues in JAK2, STAT1, TYK2, and SOCS1 in chicken cell lines.