Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/29/2017
Publication Date: 11/29/2017
Citation: Jiao, Y., Burke, J.J., Chuang, K.J., Burow, P.D., Chen, J., Hayes, C.M., Emendack, Y., Ware, D., Xin, Z. 2017. Sequenced sorghum mutant library- an efficient platform for discovery of causal gene mutations [abstract]. Meeting Abstract. Paper No. 001.
Technical Abstract: Ethyl methanesulfonate (EMS) efficiently generates high-density mutations in genomes. We applied whole-genome sequencing to 256 phenotyped mutant lines of sorghum (Sorghum bicolor L. Moench) to 16x coverage. Comparisons with the reference sequence revealed >1.8 million canonical EMS-induced G/C to A/T mutations, 22% of which were in genic regions, affecting >95% of genes in the sorghum genome. The vast majority (97.5%) of the induced mutations were distinct from natural variations. By applying the mutation database to phenotype analysis, we identified four causal gene mutations affecting drought tolerance, two mutations affecting heat tolerance, and two mutations affecting seed size that corresponded to previously reported seed size QTLs. Our results demonstrate that this collection of sequenced mutant lines can be used to efficiently discover new traits and their underlying causal mutations, thereby accelerating sorghum breeding.