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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #345231

Research Project: Genetic Enhancement of Sorghum as a Versatile Crop

Location: Plant Stress and Germplasm Development Research

Title: Sequenced sorghum mutant library- an efficient platform for discovery of causal gene mutations

Author
item Jiao, Yinping
item Burke, John
item Chuang, Kaylee
item Burow, Paul
item Chen, Junping
item Hayes, Chad
item Emendack, Yves
item Ware, Doreen
item Xin, Zhanguo

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/29/2017
Publication Date: 11/29/2017
Citation: Jiao, Y., Burke, J.J., Chuang, K.J., Burow, P.D., Chen, J., Hayes, C.M., Emendack, Y., Ware, D., Xin, Z. 2017. Sequenced sorghum mutant library- an efficient platform for discovery of causal gene mutations [abstract]. Meeting Abstract. Paper No. 001.

Interpretive Summary:

Technical Abstract: Ethyl methanesulfonate (EMS) efficiently generates high-density mutations in genomes. We applied whole-genome sequencing to 256 phenotyped mutant lines of sorghum (Sorghum bicolor L. Moench) to 16x coverage. Comparisons with the reference sequence revealed >1.8 million canonical EMS-induced G/C to A/T mutations, 22% of which were in genic regions, affecting >95% of genes in the sorghum genome. The vast majority (97.5%) of the induced mutations were distinct from natural variations. By applying the mutation database to phenotype analysis, we identified four causal gene mutations affecting drought tolerance, two mutations affecting heat tolerance, and two mutations affecting seed size that corresponded to previously reported seed size QTLs. Our results demonstrate that this collection of sequenced mutant lines can be used to efficiently discover new traits and their underlying causal mutations, thereby accelerating sorghum breeding.