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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Insect Control and Cotton Disease Research » Research » Publications at this Location » Publication #343330

Research Project: COTTON DISEASE MANAGEMENT STRATEGIES FOR SUSTAINABLE COTTON PRODUCTION

Location: Insect Control and Cotton Disease Research

Title: Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Author
item Joshi, Sameer - Texas A&m University
item Kumar, Vinod - Texas A&m University
item Janga, Madhusudhana - Texas A&m University
item Bell, Alois - Al
item Rathore, Keerti - Texas A&m University

Submitted to: Physiology and Molecular Biology of Plants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/27/2016
Publication Date: 1/3/2017
Citation: Joshi, S.G., Kumar, V., Janga, M.R., Bell, A.A., Rathore, K.S. 2017. Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates. Physiology and Molecular Biology of Plants. 23:135-142.

Interpretive Summary: Transgenic cotton plants expressing the gene AtNPR1 seem to slow down the spread of Fusarium wilt, specifically the isolate Fov11, as compared to wild-type plants. Analysis of roots and stems from seedlings with AtNPR1 show that these transgenic lines are capable of launching a stronger defense when infected with Fov11. However, little or no protection was observed against a similar strain of Fusarium wilt (Fov43) or a highly-virulent one like CA-9.

Technical Abstract: In our earlier investigation, we had demonstrated that transgenic cotton plants expressing AtNPR1 showed significant tolerance to Fusarium oxysporum f. sp. vasinfectum, isolate 11 (Fov11) and several other pathogens. The current study was designed to further characterize the nature of the protection provided by AtNPR1 expression and its limitations. Green Fluorescent Protein-expressing Fov11 was generated and used to study the progression of the disease within the plant. The results show that the spread of the pathogen was slower in the AtNPR1-transformants compared to the wild type plants. Transcript analysis in the seedling root and hypocotyl showed that the transgenic lines are capable of launching a stronger defense response when infected with Fov11. We further confirmed that AtNPR1 transformants showed greater degree of tolerance to Fov11. However, little or no protection was observed against a related, but more virulent isolate, Fov43, and a highly virulent isolate, CA9.