Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/2017
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: We undertook a study to assess the effects of Allium hookeri (AH) root and fermented root on inflammation and intestinal integrity of lipopolysaccharide (LPS)-challenged chickens. Birds were assigned to six groups (n = 25 birds/treatment) and fed with basal diets or basal diets supplemented with AH root or fermented root at two concentrations (1 or 5 %). At 7 d of age, five groups (n = 125) in each dietary treatment were injected with LPS (1 mg/kg body weight), and remaining 25 birds injected with PBS as a negative control. LPS challenge significantly reduced average body weight gain at 24 h post-injection compared with PBS control. Fermented root supplementation increased average body weight gain by 1 % compared with the LPS-challenged control. Serum a-1-AGP levels, interleukin (IL)-1ß, IL-8, tumor necrosis factor superfamily member 15 (TNFSF15), and LPS-induced tumor necrosis factor-a factor (LITAF) transcript levels were significantly higher in the small intestine in LPS-injected chickens. However a-1-AGP levels were reduced by AH root or fermented root (1 and 5 %) supplementation and IL-1ß, IL-8, and LITAF were also down-regulated by root and fermented root (1 and 5 %) supplementation. The reduced expression of tight junction proteins (junctional adhesion molecule 2 (JAM2) and occludin) and intestinal mucin 2 (MUC2) by LPS challenge was reversed by root or fermented root (1 and 5 %) supplementation. These findings demonstrate that dietary AH root and fermented root influence anti-inflammatory activity and tight junction protein expression in LPS-induced chickens.