Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 8/5/2017
Publication Date: 12/31/2017
Citation: Swisher Grimm, K.D., Porter, L.D. 2017. Use of KASP markers to screen the Pisum sativum single plant core collection and commercial varieties for resistance to pea seed-borne mosaic virus pathotype P-1. Phytopathology. https://doi.org/10.1094/PHYTO-107-12-S5.1.
Interpretive Summary: Pea seedborne mosaic virus (PSbMV) can cause major economic issues in pea production areas throughout the world. The most effective and economical means of managing this pathogen is using genetic resistance to prevent the development and infection of the virus in pea plants. This research used two genetic markers to rapidly screen through 325 pea lines collected from all over the world, referred to as the Pisum Core Collection, to determine lines with resistant genes. Forty-six commercially available pea varieties were also evaluated for resistance. Twenty-two pea lines from the Pisum Core Collection were determined to have one of two different mechanisms of conferring resistance to one of the most common strains of PSbMV. Less than 11% of the commercial lines demonstrated resistance to PSbMV, and only a single type of resistance was observed in these lines. PSbMV-resistant breeding material from the Pisum Core Collection and commercial lines have now been identified that can be used in pea breeding programs to improve genetic resistance to this virus.
Technical Abstract: Kompetitive allele-specific PCR (KASP) markers were previously developed to distinguish two resistance alleles of the translation initiation factor (eIF4E) in Pisum sativum against Pea seed-borne mosaic virus (PSbMV) pathotype P-1. Endpoint genotyping was conducted using KASP markers to separate PSbMV-susceptible lines from those comprising one of the two eIF4E-resistance alleles. Using these markers, the 325 lines of the P. sativum single plant core collection (SPCC) and 46 commercial varieties were screened. Genotypes were correlated to phenotypic and serological assay results obtained for PSbMV from greenhouse trials. Of the 325 single plant lines, 22 were resistant to the PSbMV pathotype P-1 in the greenhouse, showing no visual PSbMV symptoms and testing negative for PSbMV by ELISA. Of these 22 resistant lines, 15 were identified with the eIF4E resistant W62L amino acid mutation, and seven with the eIF4E resistant S78 amino acid deletion using the KASP markers. Two SPCC lines showed a mixed population of PSbMV-susceptibility and W62L resistance, both by ELISA and KASP marker analysis. Of the 46 commercial varieties screened, five showed resistance to PSbMV pathotype P-1 in the greenhouse and tested negative by ELISA, and were all found to have the eIF4E-resistant W62L amino acid mutation based on the KASP markers. This genotyping screen of the SPCC and commercial varieties provides breeders with information regarding PSbMV-resistance in their programs.