Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 8/5/2017
Publication Date: 12/31/2017
Citation: Swisher Grimm, K.D., Porter, L.D. 2017. Development of KASP markers for the identification of pea seed-borne mosaic virus pathotype P-1 resistant alleles of eIF4E in Pisum sativum. Phytopathology. https://doi.org/10.1094/PHYTO-107-12-S5.1.
Interpretive Summary: Pea seed-borne mosaic virus (PSbMV) is an economically important plant virus that infects peas, chickpeas and lentils. The virus can be transmitted in seed and can therefore be spread easily by the movement of seed via trade. Determining pea varieties with resistance to PSbMV is critical to preventing the spread of this pathogen to growing areas throughout the world and improving yields. This research developed two genetic markers that can be used to determine whether or not a particular pea plant is susceptible or resistant to infection by one of the most common strains of PSbMV. Using these genetic markers provides an effective means for pea breeders to rapidly screen through pea lines to find resistant breeding material, instead of having to routinely screen for resistance by large, time-consuming greenhouse or field trials that require physical observations of the plants for classic symptoms associated with the virus or using antibodies in ELISA protocols to determine the presence of the pathogen in plant tissue.
Technical Abstract: Pea seed-borne mosaic virus (PSbMV) is an economically important potyvirus of legumes, specifically of pea (Pisum sativum) where it causes leaf curling, mosaic, and vein discoloration, and can reduce seed quality and quantity. Resistance of P. sativum to the P-1 pathotype of PSbMV has been mapped to the eIF4E translation initiation factor, a common resistance gene effective against different members of the family Potyviridae. Two kompetitive allele-specific PCR (KASP) markers were designed to target allelic differences previously identified in the eIF4E gene of P. sativum. The first marker targeted a single nucleotide polymorphism that generated the W62L amino acid mutation in the resistant eIF4E allele, and the second targeted an alternate resistance allele with a three-nucleotide deletion that generated the S78 amino acid deletion. These two KASP markers were effective in differentiating known susceptible P. sativum accessions from those known to have one of these two modes of eIF4E resistance to PSbMV pathotype P-1. The endpoint genotyping analysis used with the KASP markers provides a quick and effective way to identify P. sativum plants with PSbMV resistance, and provides an effective tool for plant breeders, who routinely screen for resistance to PSbMV by large, time-consuming greenhouse trials that require phenotypic and serological identification of virus resistant accessions.