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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #339622
Title: A new virus in Luteoviridae is associated with raspberry leaf curl disease

Author
item DI BELLO, PATRICK - Oregon State University
item DIAZ-LARA, ALFREDO - University Of California
item Martin, Robert

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/2/2017
Publication Date: 6/2/2017
Citation: Di Bello, P., Diaz-Lara, A., Martin, R.R. 2017. A new virus in Luteoviridae is associated with raspberry leaf curl disease. Abstract for the American Phytopathological Society; 2017 Aug 5-9; San Antonio, TX.

Interpretive Summary:

Technical Abstract: To determine the etiology of Raspberry Leaf Curl Disease (RLCD), which causes leaf curling, leaf distortion, leaf chlorosis, shoot dwarfing, shoot proliferation in raspberries and can kill plants within three years, a next generation sequences approach was applied. Two red raspberry plants collected from native habitats in Wisconsin that exhibited symptoms of RLCD were used for the NGS analysis. Total nucleic acids were extracted, digested with DNase, and after removal of polyribosomal RNA, were subjected to next generation sequencing on the Illumina Miseq platform with paired end 150 bp. Two known viruses were found Black raspberry necrosis virus (BRNV) and Rubus yellow net virus (RYNV), and two new viruses, one was Cytorhabdovirus-like and the other Luteoviridae-like. The new virus in Luteoviridae is related to viruses in the genera Enamovirus and Polerovirus. The luteo-like virus was found to be graft transmissible to raspberries and blackberries, and vectored by the large raspberry aphid, Amphorophora agathonica. In limited testing done to date, the virus was not found in commercial Rubus collected in Oregon and Washington, but was detected in symptomatic wild raspberry collected from eastern U.S.A. and Canada and in some accessions of native Rubus in the USDA-ARS NCGR Rubus collection. The full genome of this novel virus as well as a detection protocol will be presented.