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Title: Genetic fingerprinting of potato varieties from the Northwest Potato Variety Development Program

item BALI, SAPINDER - Oregon State University
item SATHUVALLI, VIDYASAGAR - Oregon State University
item Brown, Charles
item Novy, Richard - Rich
item EWING, LORIE - University Of Idaho
item DEBONS, JEANNE - Potato Variety Management Institute
item DOUCHES, DAVID - Michigan State University
item COOMBS, JOSEPH - Michigan State University
item Navarre, Duroy - Roy
item Whitworth, Jonathan
item CHARLTON, BRIAN - Oregon State University
item YILMA, SOLOMAN - Oregon State University
item SHOCK, CLINTON - Oregon State University
item STARK, JEFF - University Of Idaho
item PAVEK, MARK - Washington State University
item KNOWLES, RICK - Washington State University

Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/14/2016
Publication Date: 2/1/2017
Citation: Bali, S., Sathuvalli, V., Brown, C.R., Novy, R.G., Ewing, L., Debons, J., Douches, D., Coombs, J., Navarre, D.A., Whitworth, J.L., Charlton, B., Yilma, S., Shock, C., Stark, J., Pavek, M., Knowles, R. 2017. Genetic fingerprinting of potato varieties from the Northwest Potato Variety Development Program. American Journal of Potato Research. 94:54-63.

Interpretive Summary: In the course of development of potato clones into varieties and while using Plant Variety Protection to protect the intellectual property, data of many kinds are assembled for application for a certificate. The Plant Vareity Protection certificate is the legal means that the breeder of the new potato variety has to control seed sales and to derive income from these. The certificate is only granted if the applied for entity is different from all potato varieties previously granted certificates. Besides physical descriptors of varieties' anatomical parts of the plant, it is possible to describe the new variety with molecular markers. This is an additional tool providing greater discernment of the uniqueness of the variety. This can be useful to resolve misidentification, mixture, and misrepresentation of a variety as being other than what it is. Plant Variety Protection allows the holder of the certificate to control who is allowed to sell seed. This is a commercial matter involving the purchase of the seed by a buyer. Permission to sell seed is usually agreed upon with provisos to pay royalty to the holder of the certificate. Obviously, correct identification of a variety without doubt is paramount to making the system function correctly. Fingerprinting is a highly accurate means to identify a variety and assure that royalties are paid to the originator.

Technical Abstract: The Northwest Potato Variety Development Program using conventional breeding has successfully released more than 40 improved varieties of potato since its inception in 1983. Potato breeders rely primarily on morphological and phenotypic data for selection and breeding of potato cultivars. With the advent of new technologies based on DNA fingerprinting, novel approaches for varietal identification have been introduced which offer advantages over traditional morphological and isozyme methods. Large scale simple sequence repeat (SSR) markers and single nucleotide polymorphism (SNP) datasets have been generated for high throughput fingerprinting in potato. Forty-six potato varieties were fingerprinted using 32 SSR markers and 12,808 SNP markers (Infinium SolCAP 12K array). Of 32 SSR markers, 29 exhibited significant polymorphism across all the 46 potato varieties. A total of 143 alleles were observed with an average of 4.6 alleles per SSR marker. These markers span all 12 chromosomes of potato, with a maximum of five markers coming from chromosome VIII and minimum of one marker from chromosome VI. Based on polymorphism, ease of amplification and scoring SSR markers, STM5114, STG0016, STM5127, STG0025, STM1053, STM2030 and STI003 are best suited for fingerprinting. Out of 12808 SNPs from the Infinium SolCAP 12K array, 88.8% resulted in reliable 3 cluster diploid calling, out of which 87.8% of the markers were polymorphic and 44.2% gave reliable 5 cluster tetraploid calling, out of which 94.5% of the markers were polymorphic. Our study provided fingerprinting resources for the Northwest potato varieties and can be utilized in issues related to intellectual property rights, ownership and trademark.