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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #326865

Research Project: Functional Genomics Approaches for Controlling Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: Cytosolic DNA sensing genes expression in the intestinal mucosa of necrotic enteritis chickens

Author
item HONG, YEONG - Chung-Ang University
item Lillehoj, Hyun
item RENGARAJ, D - Chung-Ang University
item TRUONG, ANH - Chung-Ang University
item KIM, GEUN - Chung-Ang University

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/9/2016
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Poultry farms often report necrotic enteritis (NE) outbreaks as a result of the inappropriate use of antibiotics in the feed. Several studies have reported that the genes related to intestine and immune system are significantly altered in response to NE. We induce NE in two chicken lines that are resistant (line 6.3) and sensitive (line 7.2) to avian leukosis and Marek’s disease. Then, RNA-sequencing analysis was performed in the intestinal mucosa of NE induced and uninfected control birds. The differentially expressed genes in the intestinal mucosa of line 6.3 and 7.2 were investigated to know their involvement in the immune related pathways using KEGG database. Among the identified pathways in chicken, cytosolic DNA sensing (CDS) pathway was selected for further analysis. RNA-sequencing and pathway analysis identified a total of 21 genes that were involved in chicken CDS pathway and differentially expressed in the intestinal mucosa of resistant and sensitive lines. The expression of CDS genes was confirmed by qRT-PCR. We found a majority of the CDS genes were significantly altered in the intestinal mucosa of NE-induced birds of resistant and sensitive lines. Our study indicates that NE seriously affects genes involved in foreign DNA sensing mechanisms in the chicken intestine. The CDS genes identified and affected after NE treatment could be an important clue for the protective immune response to necrotic enteritis pathogens.