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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research » Research » Publications at this Location » Publication #326533

Research Project: Improved Strategies for Management of Soilborne Diseases of Horticultural Crops

Location: Horticultural Crops Research

Title: Draft transcriptome of Globodera ellingtonae

Author
item Phillips, Wendy
item EVES-VAN DEN AKKER, SEBASTIAN - University Of Dundee
item Zasada, Inga

Submitted to: Journal of Nematology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2017
Publication Date: 6/1/2017
Citation: Phillips, W.S., Eves-van den Akker, S., Zasada, I.A. 2017. Draft transcriptome of Globodera ellingtonae. Journal of Nematology. 49(2):129–130.

Interpretive Summary: Potato cyst nematodes, Globodera pallida and G. rostochiensis, are globally important nematode parasites of potato, with the potential to cause up to 80% reduction in potato yield. Recently, a new potato cyst nematode, G. ellingtonae, was described with this nematode being genetically intermediate between the other potato cyst nematodes. Research was conducted to characterize the transcriptome, otherwise known as the molecules expressed by an organism. These results are significant because they provide a framework for further understanding the genetic relationships among the potato cyst nematodes and ultimately how they interact with their host. This research will be used by scientists to continue to explore the genetics of potato cyst nematodes and may lead to the development of novel management for these production-limiting pests.

Technical Abstract: The recently described cyst nematode species, Globodera ellingtonae, is a phylogenetic intermediary between the potato cyst nematodes (PCN), G. rotochiensis and G. pallida, and as such provides a new avenue for understanding the evolution and biology of PCN. Given that assembled genomes and transcriptomes are available for both PCN, the addition of such data for G. ellingtonae will provide a unique perspective from which to compare characteristics within this lineage. The multi life stage transcriptome was assembled for G. ellingtonae. The final assembly had a GC content of 49.0% and contained 20,622,916 bases with 30,541 transcripts organized into 20,286 read clusters, putatively representing 26,901 genes, with average and median contig lengths 675 bp and 426 bp, respectively.