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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #325955

Research Project: Epidemiology and Management of Pierce's Disease and Other Maladies of Grape

Location: Crop Diseases, Pests and Genetics Research

Title: The complete mitochondrial genome sequence of Diaphorina citri (Hemiptera: Psyllidae)

Author
item Wu, F - South China Agricultural University
item Cen, Y - South China Agricultural University
item Deng, X - South China Agricultural University
item Zheng, Z - South China Agricultural University
item Chen, Jianchi
item Liang, G - South China Agricultural University

Submitted to: Mitochondrial DNA
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/17/2016
Publication Date: 3/29/2016
Citation: Wu, F., Cen, Y., Deng, X., Zheng, Z., Chen, J., Liang, G. 2016. The complete mitochondrial genome sequence of Diaphorina citri (Hemiptera: Psyllidae). Mitochondrial DNA. 1:239-240. doi: 10.1080/23802359.2016.1156491.

Interpretive Summary: Asian citrus psyllid (Diaphorina citri) is an important pest that transmits the pathogen of Huanglongbing (HLB), a highly destructive disease affecting citrus production worldwide. For effective management strategies, knowledge of the insect population diversity is needed. For this purpose, the mitochondrial genome of an Asian citrus psyllid vector from Guangdong, China, where HLB was first described, was sequenced. This is the first complete mitogenome sequence of Asian citrus psyllid that will serve as a reference to identify genetic markers for future population studies.

Technical Abstract: The first complete mitochondrial genome (mitogenome) sequence of Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae), from Guangzhou, China is presented. The circular mitogenome is 14,996 bp in length with an A+T content of 74.5%, and contains 13 protein-coding genes (PCGs), 22 tRNA genes (tRNAs), 2 rRNA genes, and an AT-rich control region. Synteny was identical to the ancestral insect mitogenomes. All PCGs start with “ATN” codons. Eleven PCGs use the complete termination codons (“TAA” or “TAG”), while the remaining two (cox2 and nad5) use a single “T” as the stop codon. All tRNAs have the typical cloverleaf structure, except for tRNA(trp) which lacks the variable arm and tRNA(ser) (AGN) which lacks the dihydrouridine arm. A phylogenetic tree, including twelve members of Sternorrhyncha, was constructed based on the 13 PCGs sequences. The resulting phylogenetic tree matched the established taxonomic scheme based on morphology.