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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #323040

Research Project: Functional Genomics Approaches for Controlling Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: In vitro Clostridium perfringens and Escherichia coli toxin adsorption of Varium

Author
item Lim, Yeaseul - U.s. Department Of Agriculture (USDA)
item Ching, San - Amlan International
item Johnston, Leann - Amlan International
item Chi, Fang - Amlan International
item Lillehoj, Hyun

Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: 12/2/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Enteric disease agents, such as Clostridium perfringens and Escherichia coli, produce detrimental biotoxins that cause significant economic loss annually in the poultry industry. The objective of this study was to determine the in vitro biotoxin adsorption capability of Varium. An enzyme-linked immunosorbent assay (ELISA) was developed to evaluate several biotoxins including heat labile toxin (LTB), shiga-like toxin (Shiga), and lipopolysaccharide (LPS) from E. coli; and a-toxin and NetB toxin from C. perfringens. To do this Varium and the toxins were incubated at several concentrations. They were then centrifuged to remove the Varium-bound toxin and the supernatant was collected and analyzed to determine the optimum toxin concentration. Binding percentage was calculated as: 100% x (total concentration - supernatant concentration)/total concentration. For Varium binding capacity to C. perfringens produced toxins, the binding percentage increased significantly (P < 0.01) from 46 to 96% when the Varium:a-toxin ratio increased from 25:1 to 100:1. The R-Square of the quadratic response was 0.98 with the equation: Binding % = 34.66 + 0.716 x Ratio - 0.0058 x (Ratio-58.33)2. Varium binding capacity for NetB toxin was 100% as the ratio increased from 25:1 to 100:1. This indicates that Varium has strong binding capacities for these two toxins that cause necrotic enteritis in poultry. For E. coli produced toxins, the binding percentage increased from 19 to 83% as the Varium:LPS ratio increased from 50:1 to 400:1. Varium can bind with LTB from 67 to 100% as the ratio went from 4:1 to 32:1. Furthermore, Varium bound Shiga from 58 to 100% with ratios from 8:1 to 32:1. These results indicate that Varium has strong binding capacities with each E. coli toxins, although more so with the exotoxins LTB and Shiga toxins than the endotoxin LPS. In conclusion, these binding tests indicate that Varium can bind with toxins from C. perfringens and E. coli.