|CLARK, JOHN - University Of Arkansas|
|PEACE, CAMERON - Washington State University|
|IEZZONI, AMY - Michigan State University|
Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/10/2015
Publication Date: 7/1/2015
Citation: Bassil, N.V., Finn, C.E., Clark, J.R., Peace, C., Iezzoni, A. 2015. Development of a multiplexed fingerprinting set in blackberry. In: Proceedings of the International Rubus and Ribes Symposium, June 18-24, 2015, Asheville, North Carolina.
Technical Abstract: A reliable and fast method for confirming identity and paternity in blackberry is needed. Microsatellite markers or simple sequence repeats (SSRs) are ideal for cultivar fingerprinting, paternity testing and identity certification. The objective of this study was to develop a multiplexed fingerprinting set composed of trinucleotide-containing SSRs and to use them to confirm parentage in breeding populations. These populations were selected from the eastern US germplasm at the Univ. of Arkansas and western germplasm in the USDA-ARS Oregon breeding programs. Thirteen mapped trinucleotide-containing SSRs were used to screen a panel of 13 diverse blackberry cultivars from the USDA-ARS National Clonal Germplasm Repository blackberry collection. Eight SSRs that were easy to score and generated 6-10 alleles each in these 13 cultivars were selected to make up the fingerprinting set. They were amplified and optimized in two PCR multiplexes. The eight SSRs differentiated between each of the 13 blackberry cultivars and generated the same profile whether they were amplified singly or in one of the multiplexes. We will report their use in paternity confirmation of Eastern and Western blackberry breeding populations.