Author
MANO, E - Universidad De Sao Paulo | |
MINAMI, S - Universidad De Sao Paulo | |
Loper, Joyce | |
ARAUJO, W - Universidade Federal De Viçosa |
Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 5/31/2015 Publication Date: 6/9/2015 Citation: Mano, E.T., Minami, S.N., Loper, J.E., Araujo, W.L. 2015. First description of necrosis in leaves and pseudo-bulbs of Oncidium orchids caused by Burkholderia gladioli in São Paulo State, Brazil. Plant Disease. doi: 10.1094/PDIS-03-15-0297-PDN. Interpretive Summary: A necrosis of orchid leaves and pseudobulbs was observed in a commercial orchid nursery in Mogi das Cruzes, Sao Paulo, Brazil. The symptoms were water-soaked, brown lesions that can develop into large areas of necrosis that extend throughout the entire plant, ultimately causing death. Bacteria were isolated from symptomatic plants, purified, and nine isolates were used to fulfill Koch's postulates. The bacteria were identified as Burkholderia gladioli based on 16S rRNA gene sequence and a range of physiological tests. The flower market in Brazil is estimated at US $2.5 billion in 2012. Considering the importance of orchids in the annual sales, future studies should be focused on disease incidence, pathogen distribution and the management of the orchid necrosis caused by B. gladioli in commercial nurseries. Technical Abstract: A necrosis of orchid leaves and pseudobulbs was observed in a commercial orchid nursery in Mogi das Cruzes, Sao Paulo, Brazil. The symptoms were water-soaked, brown lesions that can develop into large areas of necrosis that extend throughout the entire plant, ultimately causing death. Bacteria were isolated from symptomatic plants, purified, and nine isolates were used to fulfill Koch's postulates. The bacteria were identified as Burkholderia gladioli based on 16S rRNA gene sequence and a range of physiological tests. |