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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #315214

Research Project: Epidemiology and Management of Pierce's Disease and Other Maladies of Grape

Location: Crop Diseases, Pests and Genetics Research

Title: Whole genome sequencing and analyses of Xylella fastidiosa subsp. fastidiosa strain GV156 causing Pierce’s disease of grapevine in Taiwan

Author
item Su, C. - Taiwan Agricultural Chemicals And Toxic Substances Research Institute
item Deng, W. - National Chung-Hsing University
item Shih, H - Taiwan Agricultural Chemicals And Toxic Substances Research Institute
item Jan, F - National Chung-Hsing University
item Chang, C - University Of Georgia
item Chen, Jianchi

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2015
Publication Date: 11/1/2015
Citation: Su, C.C., Deng, W.L., Shih, H.T., Jan, F.J., Chang, C.J., Chen, J. 2015. Whole genome sequencing and analyses of Xylella fastidiosa subsp. fastidiosa strain GV156 causing Pierce’s disease of grapevine in Taiwan. American Phytopathological Society Annual Meeting. 105:S4.133.

Interpretive Summary:

Technical Abstract: Xylella fastidiosa is a nutritionally fastidious Gram-negative bacterium causing Pierce’s disease (PD) of grapevines. PD was first reported in Anaheim, California in 1892 and is currently endemic in California and the southeastern U.S. PD also was found outside the U.S. but is limited to the Americas with the exception of Kosovo in Europe and Taiwan in Asia. In Taiwan, PD-like symptoms, such as irregular scorching on leaf margins, matchstick-like petioles on canes, and patchy bark symptoms on stems (green islands), were observed in a vineyard in Nantou County in 2002. Further research based on bacterial culture, pathogenicity tests, and 16S rDNA sequence signature indicated that the causal agent was a strain of Xylella fastidiosa. This study further characterized the Taiwan PD bacterium by whole genome sequencing and analyses of a representative strain (GV156). The strain was first triple-cloned in PD2 medium. Total genomic DNA was extracted and sequenced using the Illumina MidSeq platform. Using the whole-genome sequence of X. fastidiosa subsp. fastidiosa Strain M23 as a reference, the GV156 genome was determined to be 2,521,639 bp. Comparison of sequences of five housekeeping genes, ssr (16S rRNA), gyrB (DNA gyrase subunit B), dnaK (chaperone protein), rpoD (RNA polymerase sigma factor), and tonB (outer membrane receptor), showed that GV156 was 100% identical to all five strains of X. fastidiosa subsp. fastidiosa with whole genome sequences available in the GenBank database. However, comparison with highly variable genomic loci such as pspB revealed variations between the Taiwan PD strain and North American PD strains. Further characterization of the Taiwan PD strain is underway.