Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2015
Publication Date: 11/1/2015
Citation: Wallis, C.M., Chen, J. 2015. Fatty acid profiling to characterize California strains of Xylella fastidiosa. Phytopathology. 105(S4):143-144.
Technical Abstract: Different strains of Xylella fastidiosa cause diseases such as Pierce’s disease of grapevine, citrus variegated chlorosis, and bacterial leaf scorch of hardwoods. However, more research is needed to better define subspecies and strains of X. fastidiosa to improve both regulations concerning this bacterium and management of diseases it causes. Traditionally, cell membrane fatty acid profiling has proven to be useful as a phenotypical characteristic to define bacterial species and subspecies. This study was conducted to develop fatty acid profiling techniques for X. fastidiosa to complement ongoing genome-based approaches to better distinguish subspecies and strains. Results have verified differences in fatty acid composition between X. fastidiosa ssp. fastidiosa and ssp. multiplex. There also was some evidence suggesting greater diversity than already established for X. fastidiosa based upon additional, smaller-scale differences in fatty acid composition which were correlated with location and host of isolation. Completion of this research will improve understanding about X. fastidiosa evolution, with potential impact on regulatory efforts and management of X. fastidiosa-caused diseases.