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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #314707

Research Project: Enhancing Plant Resistance to Water-Deficit and Thermal Stresses in Economically Important Crops

Location: Plant Stress and Germplasm Development Research

Title: Identification and confirmation of root-knot nematode and Fusarium wilt disease resistance traits in cotton substitution lines

item WANG, CONGLI - University Of Southern California
item Ulloa, Mauricio
item Saha, Sukumar
item STELLY, DAVID - Texas A&M University
item Jenkins, Johnie
item ROBERTS, PHILIP - University Of Southern California

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/5/2015
Publication Date: 8/5/2015
Citation: Wang, C., Ulloa, M., Saha, S., Stelly, D.M., Jenkins, J.N., Roberts, P. 2015. Identification and confirmation of root-knot nematode and Fusarium wilt disease resistance traits in cotton substitution lines [abstract]. The American Phytopathological Society (APS) Annual Meeting, August 1-5, 2015, Pasadena, California. P83-0.

Interpretive Summary:

Technical Abstract: A recombinant inbred line population between Upland Gossypium hirsutum TM-1 and Pima G. barbadense 3-79 was previously used to identify QTL determining response to both root-not nematode and Fusarium wilt races 1 (FOV1) and 4 (FOV4), an economically important diseases in cotton. To confirm QTLs and to identify new QTLs, 32 cotton chromosome substitution (CS) lines developed in the TM-1 background including 25 CS-B lines from the 3-79 donor were used to examine phenotypic response to both diseases in inoculation assays. Three CS-B lines (04, 22sh, 18) had lower nematode-induced root-galling indices than TM-1, 3-79 and other CS lines. The previously identified QTLs Mi-GIb-C04 and Mi-Glb-C22 associated with root-galling response in 3-79 were located in the substitution regions of CS-B04 and 22sh, indicating that these 3-79 substitution chromosomes caused higher resistance. CS-B14sh supported greater nematode egg numbers due to 3-79 chromosome introgression, which agreed with QTL Mi-EGRh-C14sh association and egg production in TM-1. In the FOV1 test, CS-B16 had less severity of wilt disease symptoms than the TM-1 parent, which confirmed the major FOV1 resistance QTL FOV1-C16 in 3-79. CS-BNTN17-11 and CS-B17 showed greater FOV4 infection symptoms, which confirmed the localization on chromosome 17 of an additional FOV4 resistance gene from Upland TM-1. This work demonstrated that CS lines are a valuable genetic resource for localizing important disease resistance trait determinants in cotton.