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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #313981

Research Project: Functional Genomics Approaches for Controlling Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: Development and characterization of mouse monoclonal antibodies reactive with chicken IL-8

Author
item Lillehoj, Hyun
item KIM, JI EUN - US Department Of Agriculture (USDA)
item LIM, YAESEUL - US Department Of Agriculture (USDA)
item JUNG, MISUN - US Department Of Agriculture (USDA)
item SULLIVAN, YVONNE - Kingfisher Biotech, Inc
item LEE, SUNG HYUN - US Department Of Agriculture (USDA)

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/5/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Interleukin-8 (IL-8) is a key mediator in neutrophil-mediated acute proinflammatory response and activates multiple signaling pathways downstream of two receptors (CXCR1 and CXCR2). However, there have not been any monoclonal antibodies which specifically detect chicken IL8 (chIL8) and define its biochemical and immunological properties. Therefore, our laboratory characterized and developed three mouse monoclonal antibodies (mAbs) which detect chIL8 by ELISA, Western blot,and Luminex assay. Using Western blot analysis, mAb specific for chIL8 identified a 9.5kDa Pichia pastgoris-expressed chIL8 and a 32kDa E.coli-derived, poly-histidine-tagged fusion protein of chIL8. The mAbs were used to measure serum IL8 levels in normal and necrotic enteritis (NE)-afflicted chickens by antigen capture ELISA and blocked the cytotoxic effect of chIL8 against lymphoblastoid chicken B tumor cells (LSCC-RP9). These new mAbs that detect chIL8 will be important immune reagents for basic and applied research in poultry immunology.