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ARS Home » Northeast Area » Frederick, Maryland » Foreign Disease-weed Science Research » Research » Publications at this Location » Publication #312713

Research Project: Identification, Characterization, and Biology of Foreign and Emerging Viral and Bacterial Plant Pathogens

Location: Foreign Disease-weed Science Research

Title: Draft genome sequence of XANTHOMONAS ARBORICOLA strain 3004, causal agent of bacterial disease on barley

item Ignatov, A
item Kyrova, E
item Vo Thi, Ngoc
item Dzhalilov, F
item Vinogradova, S
item Kamionskaya, A
item Schaad, Norman
item Luster, Douglas - Doug

Submitted to: Genome Announcements
Publication Type: Research Notes
Publication Acceptance Date: 1/8/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary: XANTHOMONAS ARBORICOLA is a species of bacteria causing symptoms of streak (bacterial blight) on specific host plants such as barley. We report here the annotated genome sequence of X. ARBORICOLA strain 3004, isolated from leaves of infected barley plants. A team of scientists from the ARS, Foreign Disease - Weed Science Research Unit, Ft. Detrick, MD, the Russian Research Institute of Phytopathology, the Peoples Friendship University of Russia, and the Centre for Bioengineering, Russian Academy of Sciences sequenced the genome of X. ARBORICOLA strain 3004 to improve our understanding of mechanisms of disease and the evolution of the genus XANTHOMONAS. The results will provide valuable information to crop breeders in development of new cultivars resistant to the bacterial pathogen.

Technical Abstract: We report here the annotated genome sequence of XANTHOMONAS ARBORICOLA str. 3004, a Gram-negative phytopathogenic bacteria that includes several pathovars characterized by virulence specificity. Strain 3004 was isolated from barley leaves with symptoms of streak (bacterial blight) and also can infect other plant species. Sequencing was performed using a whole-genome strategy employing shotgun and paired-end genome libraries (178.5 Mb). Shotgun and paired-end reads were assembled into 132 contigs from 98 173 to 528 b.p. The total size of the assembled genome is 4,765,897 bp, with a G/C content of 65.3 %. Whole-genome nucleotide comparison of the draft genome of X. ARBORICOLA strain 3004 using BLASTn indicated 99% identity to draft genome sequences of other sequenced strains of X. ARBORICOLA. The annotation for the str. 3004 genome sequence detected 4,113 coding sequences, 2 rRNAs, and 55 RNAs. The sequenced, annotated genome of X. ARBORICOLA strain 3004 will improve our understanding of molecular mechanisms of pathogenesis and evolution of the genus XANTHOMONAS.