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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #312351

Research Project: Epidemiology and Management of Pierce's Disease and Other Maladies of Grape

Location: Crop Diseases, Pests and Genetics Research

Title: Increased detection sensitivity of HLB pathogen by targeting a multi-copy gene

Author
item Chen, Jianchi
item Zheng, Z - South China Agricultural University
item Deng, X - South China Agricultural University

Submitted to: Citrograph
Publication Type: Popular Publication
Publication Acceptance Date: 2/15/2015
Publication Date: 3/20/2015
Citation: Chen, J., Zheng, Z., Deng, X. 2015. Increased detection sensitivity of HLB pathogen by targeting a multi-copy gene. Citrograph. p.44-49.

Interpretive Summary:

Technical Abstract: Citrus huanglongbing (HLB, yellow shoot disease) is a highly destructive disease in citrus production worldwide. “Candidatus Liberibacter asiaticus” is the pathogen of HLB based on strong evidence linking this bacterium with the disease. A single incidence of HLB has been reported in California. Although the infected tree was quickly destroyed, continuing efforts for early pathogen detection are crucial for the California HLB control program. A new technique for early HLB diagnosis is being developed. A multi-copy gene in the HLB pathogen genome has been identified. By targeting this bacterial gene, a highly sensitive and accurate detection protocol has been developed. Further optimization of the protocol using the new assay is underway.