|Zheng, Z - South China Agricultural University|
|Deng, X - South China Agricultural University|
Submitted to: Citrograph
Publication Type: Popular Publication
Publication Acceptance Date: 2/15/2015
Publication Date: 3/20/2015
Citation: Chen, J., Zheng, Z., Deng, X. 2015. Increased detection sensitivity of HLB pathogen by targeting a multi-copy gene. Citrograph. p.44-49.
Technical Abstract: Citrus huanglongbing (HLB, yellow shoot disease) is a highly destructive disease in citrus production worldwide. “Candidatus Liberibacter asiaticus” is the pathogen of HLB based on strong evidence linking this bacterium with the disease. A single incidence of HLB has been reported in California. Although the infected tree was quickly destroyed, continuing efforts for early pathogen detection are crucial for the California HLB control program. A new technique for early HLB diagnosis is being developed. A multi-copy gene in the HLB pathogen genome has been identified. By targeting this bacterial gene, a highly sensitive and accurate detection protocol has been developed. Further optimization of the protocol using the new assay is underway.