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ARS Home » Northeast Area » Geneva, New York » Grape Genetics Research Unit (GGRU) » Research » Publications at this Location » Publication #311984
Title: QTL identification in an interspecific grapevine cross segregating for resistance to Powdery Mildew, Downy Mildew, Black Rot, and Phylloxera

Author
item TEH, SOON LI - University Of Minnesota
item CLARK, MATT - University Of Minnesota
item HEMSTAD, PETER - University Of Minnesota
item FRESNEDO, JONATHAN - Cornell University
item SUN, QI - Cornell University
item Cadle-Davidson, Lance
item HEGEMAN, ADRIAN - University Of Minnesota
item LUBY, JIM - University Of Minnesota

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/15/2014
Publication Date: 1/1/2015
Citation: Teh, S., Clark, M., Hemstad, P., Fresnedo, J., Sun, Q., Cadle Davidson, L.E., Hegeman, A., Luby, J. 2015. QTL identification in an interspecific grapevine cross segregating for resistance to Powdery Mildew, Downy Mildew, Black Rot, and Phylloxera. Annual International Plant & Animal Genome Conference, San Diego, CA.

Interpretive Summary:

Technical Abstract: Grapevine is a highly heterozygous plant with a complex genetic background. Here, we report the use of an F1 family (N = 125) from a cross of MN1264 × MN1246 made in 2010. The cross contains at least six Vitis species in its ancestry and segregates for resistance to powdery mildew (Erysiphe necator), downy mildew (Plasmopara viticola), black rot (Guignardia bidwellii), and foliar phylloxera (Daktulosphaira vitifoliae). Plants were rated for symptom incidence in a Minnesota vineyard in 2012, 2013, and 2014. For linkage mapping, single nucleotide polymorphism (SNP) markers were obtained by genotyping-by-sequencing with alignment to the Vitis vinifera ‘PN40024’ 12X.2 reference genome. Two parental linkage maps of 2,239 cM and 1,917 cM were constructed in JoinMap 4.1 using 1,656 and 1,378 high-quality SNPs, giving average densities of 1.35 cM/SNP and 1.39 cM/SNP, female and male maps respectively. Using R/qtl for QTL mapping, our analysis confirmed two major powdery mildew resistance QTL in the regions of the reported Ren2 and Ren3 loci on chromosome 14 and 15, respectively. We observed three putative phylloxera resistance loci on chromosomes 2, 14, and 15, as well as three putative resistance loci for black rot on chromosomes 2, 14, and 15. Our analysis also indicated two putative loci for downy mildew resistance on chromosomes 2 and 15. With the application of next-generation sequencing tools, our work demonstrated successful detection of novel QTL and validation of known QTL for resistance traits in a pedigree-based breeding population.