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Research Project: Improving Fruit Quality, Disease Resistance, and Tolerance to Abiotic Stress in Grape

Location: Grape Genetics Research

Title: An update on VitisGen: recent advances in using DNA marker technologies in U.S. grape breeding programs

item Cadle-davidson, Lance
item Reisch, Bruce
item Sun, Qi
item Schweitzer, Peter
item Londo, Jason
item Ledbetter, Craig
item Luby, Jim
item Hemstad, Peter
item Hegeman, Adrian
item Teh, Soon Li
item Manns, David
item Barba, Paola
item Hyma, Katie
item Lillis, Jacquelyn
item Fresnedo, Jonathan
item Yang, Shanshan
item Takacs, Elizabeth

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/16/2014
Publication Date: 1/1/2015
Citation: Cadle Davidson, L.E., Reisch, B., Sun, Q., Schweitzer, P., Londo, J.P., Ledbetter, C.A., Luby, J., Hemstad, P., Hegeman, A., Teh, S., Manns, D., Barba, P., Hyma, K., Lillis, J.A., Fresnedo, J., Yang, S., Takacs, E. 2015. An update on VitisGen: recent advances in using DNA marker technologies in U.S. grape breeding programs. Annual International Plant & Animal Genome Conference. W419: 14264.

Interpretive Summary:

Technical Abstract: The USDA-NIFA VitisGen project involves a multidisciplinary team of 25 co-PIs at 11 institutions aiming to optimize grape cultivar improvement. To this end, an impressive collection of phenotypic data and high-resolution genetic maps has been developed for seventeen F1 families. Here, we will present an update on a subset of our progress in adapting genotyping-by-sequencing (GBS) technology for highly heterozygous species, in the development of a Heterozygous Mapping Strategy (HetMappS) for GBS-based linkage mapping, and in the phenotyping and identification of QTL associated with diverse traits, ranging from fruit quality to pest resistance to vine morphology. We will contrast different analytical approaches we have taken and provide a roadmap for application of GBS in heterozygous species. Finally, we will discuss our experiences using other marker technologies for marker assisted selection. The results we have obtained have the potential not only to accelerate grape cultivar improvement but also to enhance our genetic understanding of Vitis spp.